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Quantitative Assessment of HSP70, IL-1ß and TNF-a in Spinal Fluid and Spinal Cord Sections of Dogs with Histopathologically Confirmed Degenerative Myelopathy and Control Dogs

Lovett, Mathew
http://rave.ohiolink.edu/etdc/view?acc_num=osu1366561072

Abstract Details

2013, Master of Science, Ohio State University, Veterinary Clinical Sciences.
Pro-inflammatory cytokines such as IL-1ß and TNF-a appear to play important roles in autoinflammation associated with amyotrophic lateral sclerosis (ALS), but underlying cellular mechanisms related to this process remain to be fully defined. Canine Degenerative myelopathy (DM) is a spontaneous large animal model of familial ALS of man and represents a valuable tool through which to evaluate the role of these pro-inflammatory cytokines in the neurodegenerative process. Here we quantify the expression of IL-1ß and TNF-a in conjunction with hsp70 (another potent stimulator of the innate immune response) in cerebrospinal fluid (CSF) and spinal cord sections from control dogs and dogs with histopathologically confirmed DM. Concentrations of hsp70, IL-1ß and TNF-a in CSF were determined using high sensitivity ELISA, and protein expression in various regions of the spinal cord was determined by immunohistochemical staining (IHC) and quantified using Aperio Scanscope (Aperio Technologies, Vista, CA). Concentrations of all three proteins were below the limit of assay detection in CSF for both normal (n=10) and DM affected dogs (n=10). Tissue analysis of IHC staining revealed enhanced hsp70 expression in ependymal cells of dogs with DM compared to controls (p=0.003). IL-1ß and TNF-a staining was reduced in white matter of DM affected dogs, likely artifactual due to negative staining created by the pathology associated with DM. Based on these findings, hsp70 could serve as an important biomarker or trigger for sustained inflammation in DM and ALS and further research in this area is warranted.
Sarah Moore (Advisor)
Joan Coates (Committee Member)
Michael Oglesbee (Committee Member)
William Fenner (Committee Member)
62 p.
Medicine; Neurobiology; Neurology; Neurosciences; Veterinary Services
canine degenerative myelopathy; neurodegeneration; heat shock protein; als

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Citations

  • Lovett, M. (2013). Quantitative Assessment of HSP70, IL-1ß and TNF-a in Spinal Fluid and Spinal Cord Sections of Dogs with Histopathologically Confirmed Degenerative Myelopathy and Control Dogs [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366561072

    APA Style (7th edition)

  • Lovett, Mathew. Quantitative Assessment of HSP70, IL-1ß and TNF-a in Spinal Fluid and Spinal Cord Sections of Dogs with Histopathologically Confirmed Degenerative Myelopathy and Control Dogs. 2013. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1366561072.

    MLA Style (8th edition)

  • Lovett, Mathew. "Quantitative Assessment of HSP70, IL-1ß and TNF-a in Spinal Fluid and Spinal Cord Sections of Dogs with Histopathologically Confirmed Degenerative Myelopathy and Control Dogs." Master's thesis, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366561072

    Chicago Manual of Style (17th edition)

osu1366561072
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This open access ETD is published by The Ohio State University and OhioLINK.