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Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library

Kunys, Andrew Richard

Abstract Details

2013, Master of Science, Ohio State University, Chemistry.
Src homology 2 Domains (SH2 domains) are modular protein binding domains which recognize a phosphotyrosine and the residues adjacent to the phosphotyrosine. Although they are known to bind to specific phosphopeptides motifs, the exact peptide binding motifs for each of the 120 human SH2 domains are not known. These proteins are associated mainly with cellular signaling affecting processes as disparate as apoptosis and proliferation. In this work, high quality specificity data was obtained from 18 different SH2 domains by screening them individually against a One-Bead-One-Peptide library. Interestingly, no two domains have the same specificity, suggesting that subtle differences mark each domain as unique. Further, most of the domains studied recognize multiple different classes of binding peptides, which implies their role in binding may be more complicated that previously realized. The data obtained is unique, in that no other method can produce data of high enough quality to unambiguously establish the subtle specificity patterns of each domain or decipher the data obtained from a domain which can recognize multiple peptide ligand classes. This data will provide insight into the basic biology of cell signaling and should prove to be a valuable resource to researchers studying in this area.
Dehua Pei, Dr. (Advisor)
Ross Dalbey, Dr. (Committee Member)
99 p.

Recommended Citations

Citations

  • Kunys, A. R. (2013). Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732

    APA Style (7th edition)

  • Kunys, Andrew. Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library. 2013. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732.

    MLA Style (8th edition)

  • Kunys, Andrew. "Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library." Master's thesis, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732

    Chicago Manual of Style (17th edition)