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Master Thesis (Final).pdf (5.22 MB)
ETD Abstract Container
Abstract Header
Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library
Author Info
Kunys, Andrew Richard
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732
Abstract Details
Year and Degree
2013, Master of Science, Ohio State University, Chemistry.
Abstract
Src homology 2 Domains (SH2 domains) are modular protein binding domains which recognize a phosphotyrosine and the residues adjacent to the phosphotyrosine. Although they are known to bind to specific phosphopeptides motifs, the exact peptide binding motifs for each of the 120 human SH2 domains are not known. These proteins are associated mainly with cellular signaling affecting processes as disparate as apoptosis and proliferation. In this work, high quality specificity data was obtained from 18 different SH2 domains by screening them individually against a One-Bead-One-Peptide library. Interestingly, no two domains have the same specificity, suggesting that subtle differences mark each domain as unique. Further, most of the domains studied recognize multiple different classes of binding peptides, which implies their role in binding may be more complicated that previously realized. The data obtained is unique, in that no other method can produce data of high enough quality to unambiguously establish the subtle specificity patterns of each domain or decipher the data obtained from a domain which can recognize multiple peptide ligand classes. This data will provide insight into the basic biology of cell signaling and should prove to be a valuable resource to researchers studying in this area.
Committee
Dehua Pei, Dr. (Advisor)
Ross Dalbey, Dr. (Committee Member)
Pages
99 p.
Subject Headings
Biochemistry
;
Bioinformatics
;
Biology
;
Chemistry
Keywords
SH2 domains, Protein-Protein Interactions, Combinatorial Chemistry, Cell Signalling, Tyrosine Phosphorylation, One-Bead-One-Compound, Partial Edman Degradation, Peptide,
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Citations
Kunys, A. R. (2013).
Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library
[Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732
APA Style (7th edition)
Kunys, Andrew.
Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library.
2013. Ohio State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732.
MLA Style (8th edition)
Kunys, Andrew. "Binding Specificity of SH2 Domains Revealed by a Combinatorial Peptide Library." Master's thesis, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374185732
Chicago Manual of Style (17th edition)
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Document number:
osu1374185732
Download Count:
640
Copyright Info
© 2013, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.