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Michael J Wenstrup - MS Thesis.pdf (1.3 MB)

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Application of Portable and Benchtop Mid-Infrared Spectrometers in Profiling Composition and Quality of Edible Oils

Wenstrup, Michael J
http://rave.ohiolink.edu/etdc/view?acc_num=osu1376922152

Abstract Details

2013, Master of Science, Ohio State University, Food Science and Technology.
The application of infrared spectroscopy techniques to problems in the food and beverage industry has yielded many novel methods. With the increase in computational power, multivariate analysis has brought the unique properties of mid-infrared (MIR) systems to the forefront. The distinct, high intensity MIR absorption bands are well-suited to authentication, classification and quantification of components in complex matrices. This technique has been applied to a variety of food systems, with particular success in the analysis of edible oils. Methods for trans-fat determination, monitoring of oxidative indices, and discrimination of adulterated oils represent only a few of the developments in field of oils. The objective of this study was to utilize MIR spectroscopy in the analysis and characterization of products and processes related to edible oils. A temperature-controlled, ZnSe ATR sampling accessory was used with a benchtop FT-IR system to monitor oxidative changes in frying oils under the influence of a patented anti-oxidation device. In addition, reference methods for free fatty acids (FFA), anisidine value (AV), and color were used to monitor oil stability. FT-IR combined with chemometrics showed differences between control and treatment, with discrimination provided by regions associated with fatty acid hydrolysis and oxidation products. As compared to the control treatment, the anti-oxidation technology decreased the rate of FFA and aldehyde formation, as well as showing a marked effect on total color difference (¿E). Overall, our results showed that a patented induction device slows the rate of lipid degradation, resulting 7-20% reduction in formation rate of key quality parameters and significantly longer utilization of frying oil. Omega-3 dietary supplements have been linked with health benefits due to their contents of EPA and DHA. The concentration of these active components in fish is highly variable; species, catch location, environment, season and processing parameters are all factors in determining the ratio of fatty acids found in the oil. Our objective was to characterize the composition of commercial Omega-3 dietary supplements using mid-infrared spectroscopy, gas chromatography, and chemometrics. Twenty-nine fish body oil (FBO), cod liver oil (CLO), and flaxseed supplements were purchased from retailers. Fatty acid composition of oils, determined by GC-FAME, encompassed a wide range of fatty acid profiles and delivery methods. Mid-infrared spectral data was collected on portable infrared systems and the spectra were used to classify supplements using SIMCA, a pattern recognition technique. In addition, PLSR was used to correlate the spectra with GC-FAME results. SIMCA analysis allowed for tight clustering of fish oil supplements into distinct classes, dependent upon the source and processing. Discriminating power showed a strong influence of the 1038 cm-1 band, which is typically associated with C-O stretching vibrations. PLSR generated multivariate models with high correlation (R = 0.99) between the infrared spectrum and fatty acid composition, and SECV of 1.74 and 1.89 for EPA and DHA, respectively. Our results indicate that ATR FT-IR spectroscopy combined with pattern recognition analysis provides for rapid, robust screening and characterization of edible oils.
Luis Rodriguez-Saona, Dr (Advisor)
John Litchfield, Dr (Committee Member)
Michael Mangino, Dr (Committee Member)
70 p.
Food Science
FT-IR; edible oils; fish oils; frying; infrared; spectroscopy; multivariate analysis; food science; mid-infrared

Recommended Citations

Citations

  • Wenstrup, M. J. (2013). Application of Portable and Benchtop Mid-Infrared Spectrometers in Profiling Composition and Quality of Edible Oils [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1376922152

    APA Style (7th edition)

  • Wenstrup, Michael. Application of Portable and Benchtop Mid-Infrared Spectrometers in Profiling Composition and Quality of Edible Oils. 2013. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1376922152.

    MLA Style (8th edition)

  • Wenstrup, Michael. "Application of Portable and Benchtop Mid-Infrared Spectrometers in Profiling Composition and Quality of Edible Oils." Master's thesis, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1376922152

    Chicago Manual of Style (17th edition)

osu1376922152
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This open access ETD is published by The Ohio State University and OhioLINK.