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Electrospun Polycaprolactone Scaffolds for Small-Diameter Tissue Engineered Blood Vessels

Lee, Carol Hsiu-Yueh
http://rave.ohiolink.edu/etdc/view?acc_num=osu1376924954

Abstract Details

2013, Doctor of Philosophy, Ohio State University, Biomedical Engineering.
Cardiovascular disease is the leading cause of death in the United States with many patients requiring coronary artery bypass grafting. The current standard is using autografts such as the saphenous vein or intimal mammary artery, however creating a synthetic graft could eliminate this painful and inconvenient procedure. Large diameter grafts have long been established with materials such as Dacron® and Teflon®, however these materials have not proved successful in small-diameter (< 6 mm) grafts where thrombosis and intimal hyperplasia are common in graft failure. With the use of a synthetic biodegradable polymer (polycaprolactone) we utilize our expertise in electrospinning and femtosecond laser ablation to create a novel tri-layered tissue engineered blood vessel containing microchannels. The benefits of creating a tri-layer is to mimic native arteries that contain an endothelium to prevent thrombosis in the inner layer, aligned smooth muscle cells in the middle to control vasodilation and constriction, and a mechanically robust outer layer. The following work evaluates the mechanical properties of such a graft (tensile, fatigue, burst pressure, and suture retention strength), the ability to rapidly align cells in laser ablated microchannels in PCL scaffolds, and the biological integration (co-culture of endothelial and smooth muscle cells) with electrospun PCL scaffolds. The conclusions from this work establish that the electrospun tri-layers provide adequate mechanical strength as a tissue engineered blood vessel, that laser ablated microchannels are able to contain the smooth muscle cells, and that cells are able to adhere to PCL fibers. However, future work includes adjusting microchannel dimensions to properly align smooth muscle cells along with perfect co-cultures of endothelial and smooth muscle cells on the electrospun tri-layer.
John Lannutti (Advisor)
Heather Powell (Advisor)
Keith Gooch (Committee Member)
Biomedical Engineering; Biomedical Research; Materials Science; Polymers
biomaterials, electrospinning, polycaprolactone, tissue engineered blood vessel, femtosecond laser ablation

Recommended Citations

Citations

  • Lee, C. H.-Y. (2013). Electrospun Polycaprolactone Scaffolds for Small-Diameter Tissue Engineered Blood Vessels [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1376924954

    APA Style (7th edition)

  • Lee, Carol. Electrospun Polycaprolactone Scaffolds for Small-Diameter Tissue Engineered Blood Vessels . 2013. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1376924954.

    MLA Style (8th edition)

  • Lee, Carol. "Electrospun Polycaprolactone Scaffolds for Small-Diameter Tissue Engineered Blood Vessels ." Doctoral dissertation, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1376924954

    Chicago Manual of Style (17th edition)

osu1376924954
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© 2013, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.