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Plant-herbivore interaction of ethylene- insensitive petunias and western flower thrips Frankliniella occidentalis (Pergande)

Kuniyoshi, Claudia H
http://rave.ohiolink.edu/etdc/view?acc_num=osu1376966622

Abstract Details

2013, Doctor of Philosophy, Ohio State University, Entomology.
The plant hormone ethylene regulates a variety of physiological and developmental processes important for plant survival, including fruit ripening, abscission, senescence and responses to biotic and abiotic stresses. Plants increase their ethylene production to trigger senescence, or in response to herbivore and pathogen attack. Ethylene insensitive petunias, Petunia x hybrida transformed with 35S::etr1-1, line 44568 (etr1-1) were developed to produce longer lasting flowers but the impact of herbivores, especially to western flower thrips (WFT), on these plants is unknown. The WFT is an important pest in flowering plants and vegetable crops worldwide. They cause significant damage to the foliage, flowers and fruits decreasing plant production and value. Resistant varieties, based on ethylene induction have been shown to be an alternative to manage WFT. Assays using whole petunia plants or individual flowers of wild-type Petunia x hybrida `Mitchell Diploid’ (MD) and etr1-1 petunias showed that WFT infestation induced ethylene production from petunia flowers. WFT laid more eggs on etr-1 petunias flowers compared to MD petunia flowers, suggesting that etr1-1 petunia flowers are better host for thrips oviposition. Bioassays were performed using individual WFT females or larvae on leaves and corollas. Oviposition did not differ between etr1-1 and MD petunia on leaves. However, on corollas WFT showed higher oviposition and lower egg and larvae mortality on etr1-1 vs MD petunias. Thrips oviposition rate and fecundity was higher on etr1-1 petunia corollas compared to MD corollas. Life-table analysis indicated that etr1-1 petunia corollas are better for WFT performance than MD petunia corollas. In addition, etr1-1 and MD petunia corollas response to thrips attack was evaluated by measuring changes in primary (amino acids) and secondary (phenolics) metabolism between etr1-1 and MD petunia corollas. Petunia corollas increased in total non-essential amino acids after WFT attack on MD corollas but not on etr1-1 corollas. Levels of total phenolics did not change in MD corollas fed on by WFT, but they increased in etr1-1 corollas. In summary, ethylene-insensitive, etr1-1, transgenic petunias are more susceptible to WFT than are MD petunia plants. Western flower thrips fitness was higher in etr1-1 petunia corollas compared to MD petunia corollas. This study demonstrated that ethylene perception plays a role on the constitutive resistance of petunia against herbivores and that WFT mediates changes in the biochemical profiles of petunia corollas.
Luis Canas, Ph.D. (Advisor)
141 p.
Entomology
ethylene, plant-insect interactions, primary metabolism, secondary metabolism, life-history traits

Recommended Citations

Citations

  • Kuniyoshi, C. H. (2013). Plant-herbivore interaction of ethylene- insensitive petunias and western flower thrips Frankliniella occidentalis (Pergande) [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1376966622

    APA Style (7th edition)

  • Kuniyoshi, Claudia. Plant-herbivore interaction of ethylene- insensitive petunias and western flower thrips Frankliniella occidentalis (Pergande). 2013. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1376966622.

    MLA Style (8th edition)

  • Kuniyoshi, Claudia. "Plant-herbivore interaction of ethylene- insensitive petunias and western flower thrips Frankliniella occidentalis (Pergande)." Doctoral dissertation, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1376966622

    Chicago Manual of Style (17th edition)

osu1376966622
488
© 2013, some rights reserved.Creative Commons License Plant-herbivore interaction of ethylene- insensitive petunias and western flower thrips Frankliniella occidentalis (Pergande) by Claudia H Kuniyoshi is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.