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A phylogenetic analysis of Micobacterium tuberculosis isolates in the Columbus and Franklin County, Ohio, area.

Eichelberger, Daniel Ernest

Abstract Details

2014, Master of Science, Ohio State University, Public Health.
Tuberculosis is a tenacious disease that can be difficult to treat and represents a significant problem for the public health. Columbus and Franklin County, Ohio, are disproportionately affected by this illness. Much of the burden of disease for this area is due to tuberculosis strains that circulate largely within foreign born and homeless populations, although other individuals contribute significantly as well. For example, the 134 samples analyzed contained 83 from foreign-born individuals. Mycobacterium tuberculosis, the causative agent responsible for this disease, is robust and has a highly conserved genome. The analysis took advantage of this characteristic by utilizing 24 locus Micobacterial Interspersed Repetitive Unit (MIRU) typing. These genetic markers were directly used to construct phylogenetic trees that grouped samples through neighbor-joining algorithms. The resulting phylograms showed several notable results. Samples representing 26 unique and 2 indeterminate spoligotype families were present in the Health Department dataset; these types indicated that all 7 of the TB-lineage families utilized by TB GIMS were represented. Thirty samples in the existing dataset did not have lineage assignments prior to analysis; these were classified and determined to represent 13 spoligotype families or, equivalently, 6 TB-lineage families. Analysis of these data also illustrated 2 sets of highly similar isolates with very close phylogenetic relationships (distance measures <0.10). These sets appear to indicate genomic change among strains that have become established in this area, such as those responsible for the ii OH_20 cluster of cases. As a whole, these results paint a picture of the current state of tuberculosis in Columbus, Ohio, and illustrate 2 important points: 1) local tuberculosis cases are caused by many phylogeographic strains and 2) the similarity of genetically distinct tuberculosis samples that indicate change among endemic strains and transmission network overlap.
Judith Schwartzbaum, PhD (Advisor)
Shu-Hua Wang, MD, MPH&TM, PharmD (Committee Co-Chair)
243 p.

Recommended Citations

Citations

  • Eichelberger, D. E. (2014). A phylogenetic analysis of Micobacterium tuberculosis isolates in the Columbus and Franklin County, Ohio, area. [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1388505965

    APA Style (7th edition)

  • Eichelberger, Daniel. A phylogenetic analysis of Micobacterium tuberculosis isolates in the Columbus and Franklin County, Ohio, area. 2014. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1388505965.

    MLA Style (8th edition)

  • Eichelberger, Daniel. "A phylogenetic analysis of Micobacterium tuberculosis isolates in the Columbus and Franklin County, Ohio, area." Master's thesis, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1388505965

    Chicago Manual of Style (17th edition)