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Characterization of the Genome of Maize Chlorotic Dwarf Virus and an Associated Satellite RNA

Ge, Xin
http://rave.ohiolink.edu/etdc/view?acc_num=osu1391600232

Abstract Details

1990, Doctor of Philosophy, Ohio State University, Plant Pathology.
Maize chlorotic dwarf virus (MCDV), the type and only member of MCDV group, has not been studied at the molecular level. This paper presents some molecular characteristics of the MCDV genome and of a satellite RNA of MCDV, discovered during this study. MCDV virion contains only one species of RNA of about 13 kilobases (kB) in length. The viral RNA had a poly(A) tract at the 3' end and a genome-linked protein (VPg) of about 18 kilodaltons (kDa) presumably at the 5' end. In vitro translation showed that the RNA can direct the synthesis of a series of proteins, with the largest being about 250 kDa. Only genomic-sized MCDV RNA was found in total cellular RNA, poly(A)-containing RNA, polyribosomal RNA and double-stranded (ds) RNA samples from MCDV-infected maize. Genomic-sized MCDV RNA was the only MCDV RNA found associated with polyribosomes from MCDV-infected maize after immunoprecipitation with antibodies to MCDV capsid proteins. It is concluded that the MCDV genome is monopartite, about 13 kB in length and positive sense, and that MCDV belongs to Picornavirus supergroup. During this work, a 1-kB ssRNA species was found encapsidated in MCDV coat proteins. This particle was termed the slower sedimenting virus-like particle (SSVLP). Northern hybridization assays showed that SSVLP RNA had less than 10% homology with MCDV genomic RNA. SSVLP appeared dependent on MCDV for replication. In infected tissue, the presence of SSVLP RNA was correlated with decreases in the amounts of MCDV virions, MCDV RNA and MCDV capsid proteins. Multimeric ds SSVLP RNAs were found in maize tissue co-infected with MCDV and SSVLP. The 3' terminal structure of the RNA was a phosphate and the 5' terminal structure was not VPg. The SSVLP RNA was sequenced and no open reading frames for proteins larger than 10 kDa were found. The sequence obtained contained 1031 nucleotides and had sequence similarities to virusoids and viroids. The sequence could assume a so-called hammerhead structure. It is concluded that the SSVLP RNA, is a satellite RNA of MCDV, now named satellite MCDV (SMCDV), which probably replicates via a rolling circle mechanism.
Donald T. Gordon (Advisor)
R. E. Gingery (Committee Member)
M. D. McMullen (Committee Member)
D. J. Jackwood (Committee Member)
198 p.
Plant Pathology

Recommended Citations

Citations

  • Ge, X. (1990). Characterization of the Genome of Maize Chlorotic Dwarf Virus and an Associated Satellite RNA [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1391600232

    APA Style (7th edition)

  • Ge, Xin. Characterization of the Genome of Maize Chlorotic Dwarf Virus and an Associated Satellite RNA. 1990. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1391600232.

    MLA Style (8th edition)

  • Ge, Xin. "Characterization of the Genome of Maize Chlorotic Dwarf Virus and an Associated Satellite RNA." Doctoral dissertation, Ohio State University, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=osu1391600232

    Chicago Manual of Style (17th edition)

osu1391600232
242
© 1990, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.