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Development of a Selective Medium for Rhodococcus Equi

Sahin, Orhan
http://rave.ohiolink.edu/etdc/view?acc_num=osu1396440093

Abstract Details

1997, Master of Science, Ohio State University, Veterinary Clinical Sciences.
Rhodococcus equi is a frequent cause of chronic suppurative bronchopneumonia, lymphadenitis, and enteritis in foals, in most cases during the first four months of life. This study was carried out to develop a selective medium for the organism for its isolation from highly contaminated sources such as soil and feces of grazing herbivores. Twenty-eight clinical isolates of R. equi recovered from lesions of infected foals were tested for susceptibility to antimicrobial agents by disk diffusion and microbroth dilution susceptibility testing methods. In-vitro the most active antibiotics were gentamicin, amikacin, netilmicin, erythromycin and trimethoprim/sulphonamide combination whereas oxacillin, cloxacillin, ceftiofur, ceftazidime, aztreonam, trimethoprim, novobiocin, and nystatin were routinely impotent to tested isolates. Based on these susceptibility patterns of R. equi isolates a selective agar medium was developed. The medium contained aztreonam 100 (µg/ml, novobiocin 25 (µg/ml and nystatin 100 Units/ml with (herein referred to as IST-ANYT) or without (herein called IST-ANY) 0.005 % potassium tellurite. After the medium was proved for its selectivity for R. equi, it was tested with a number of soil and fecal samples for isolation of the organism from a Quarter Horse breeding farm in Columbus, OH. All soil samples (n=10) and fecal specimens from the rectum of healthy mares (n=12) yielded R. equi on either medium after 48 hours of incubation while the organism was not isolates from feces of any yearlings (n=4). These results show the satisfactory capacity of the new selective medium for isolation of R. equi from contaminated sources, and confirm the findings of others that the organism is widespread in equine feces and their soil environment.
Joseph J. Kowalski (Advisor)
Stephen M. Reed (Committee Member)
Kenneth W. Hinchcliff (Committee Member)
72 p.
Veterinary Services

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Citations

  • Sahin, O. (1997). Development of a Selective Medium for Rhodococcus Equi [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1396440093

    APA Style (7th edition)

  • Sahin, Orhan. Development of a Selective Medium for Rhodococcus Equi. 1997. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1396440093.

    MLA Style (8th edition)

  • Sahin, Orhan. "Development of a Selective Medium for Rhodococcus Equi." Master's thesis, Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1396440093

    Chicago Manual of Style (17th edition)

osu1396440093
3,698
© 1997, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.