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Thromboelastographic Clot Parameters of Autologous Equine Blood Products Activated by Various Clotting Agents

Ghassab, Sasan
http://rave.ohiolink.edu/etdc/view?acc_num=osu1397432210

Abstract Details

2014, Master of Science, Ohio State University, Mechanical Engineering.
Chronic musculoskeletal injuries in horses can often lead to lameness and a significant financial loss for the owner. Moreover, the healing process associated with these injuries is often slow and incomplete in tissues with poor intrinsic healing properties, such as tendons, ligaments and cartilage. Biologic products, as part of the field of regenerative medicine, can improve this healing process by introducing autologous platelet or plasma products to the site of injury. The concentration of plasma and platelets may produce an enriched product for use in regenerative medicine applications such as a spray for wounds, a gel to adhere bone grafts, or as a biological adhesive. In this study, the clotting efficiency of platelet-rich plasma (PRP) and concentrated platelet-poor plasma (cPPP) are compared to citrated whole blood when activated by each of 3 clotting agents (autologous thrombin, bovine thrombin or calcium chloride [CaCl2]) to assess the dynamic properties of fibrin and platelet bonding for surgery or injection when a holding clot is desired. Blood was drawn from 6 horses and immediately processed to generate PRP and cPPP via commercial devices. Clotting parameters were generated by a thromboelastograph. Clotting tests and a physical inspection were performed to establish normality of test subjects and to determine the platelet counts of whole blood and PRP for each horse. Whole blood, PRP, and cPPP clotted with all agents. Among blood products, PRP demonstrated the greatest clot strength and quickest clot rate. The cPPP had the weakest clot strength, slowest clot rate, and longest clot initiation time. Among clotting agents, bovine thrombin had the shortest clot initiation time, quickest clot rate and tied CaCl2 for greatest clot strength. Furthermore, CaCl2 had the longest clot initiation time and time to reach maximum clot strength. Autologous thrombin had the lowest clot strength. When combined with either bovine thrombin (rapidly) or CaCl2 (slowly), PRP provided the top combinations for clinical use. Autologous thrombin was suboptimal, but would be an autologous alternative for clinical application. As prepared here, cPPP had inefficient clotting, but may be sufficient for plasma spray indications.
Alicia Bertone (Advisor)
Manoj Srinivasan (Committee Member)
40 p.
Biology; Biomedical Research
Platelet-Rich Plasma; PRP; Concentrated Platelet-Poor Plasma; cPPP; autologous thrombin; bovine thrombin; thromboelastography; TEG; clot strength

Recommended Citations

Citations

  • Ghassab, S. (2014). Thromboelastographic Clot Parameters of Autologous Equine Blood Products Activated by Various Clotting Agents [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397432210

    APA Style (7th edition)

  • Ghassab, Sasan. Thromboelastographic Clot Parameters of Autologous Equine Blood Products Activated by Various Clotting Agents. 2014. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1397432210.

    MLA Style (8th edition)

  • Ghassab, Sasan. "Thromboelastographic Clot Parameters of Autologous Equine Blood Products Activated by Various Clotting Agents." Master's thesis, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397432210

    Chicago Manual of Style (17th edition)

osu1397432210
329
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This open access ETD is published by The Ohio State University and OhioLINK.