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Interplay between unusual MYB transcription factors and their role in cell size regulation in plants

Machemer-Noonan, Katja Marlene
http://rave.ohiolink.edu/etdc/view?acc_num=osu1397482170

Abstract Details

2014, Doctor of Philosophy, Ohio State University, Molecular Genetics.
The study of transcription factors involved in the regulation of cellular processes such as cell expansion and cell division or metabolic pathways such as biosynthesis of phenylpropanoids presents a valuable and effective approach to improve the quality of agriculturally important crops. We have identified SlFSB1 (Solanum lycopersicum Fruit SANT/MYB Binding protein1, FSB1) as a MYB transcription factor that physically interacts with two additional MYBs, SlFSM1, previously associated with tomato fruit-specific expression, and SlMYBI, a DNA-binding protein with two non-canonical MYB domains. Both proteins compete for binding to the SlFSB1 MYB domain, and these interactions can be confirmed in Arabidopsis, suggesting a similar mode of action. RNA-Seq analysis of plants ectopically expressing SlFSM1 suggests the involvement of this complex in several pathways resulting in development of smaller fruits when compared to the wild type. The interplay between the three MYBs is thought to be involved in the regulation of differential cell expansion, as overexpression of SlFSM1 results in a significant reduction of cell expansion in the tomato of cells with the highest expansion potential. Tomato fruit size is a major agronomical trait, and understanding the contribution of the studied MYBs will be greatly beneficial. Additionally, as modification of lignocellulosic biomass is becoming progressively more imperative in the generation of cost-efficient biofuels, the control of phenylpropanoid biosynthesis plays an important role in the achievement of this goal. We identified two R2R3-MYB transcription factors, ZmMYB40 and ZmMYB95, which potentially present a way of fine-tuning lignin composition in maize cell walls, in combinatorial gene expression control with two negative regulators of the phenylpropanoid pathway, ZmMYB31 and ZmMYB42. Maize plants harboring RNAi constructs against ZmMYB40 display statistically lower expression for a number of pathway genes, further supporting this hypothesis. Further, RNA-Seq of rice plants over-expressing the rice homolog of ZmMYB31 elucidates its transcriptional effect beyond those targets already identified in maize, expanding its possible impact.
Erich Grotewold (Advisor)
Venkat Gopalan (Committee Member)
David Mackey (Committee Member)
Iris Meier (Committee Member)
Rivka Barg (Committee Member)
240 p.
Agricultural Engineering; Alternative Energy; Bioinformatics; Biology; Botany; Cellular Biology; Developmental Biology; Horticulture; Molecular Biology; Plant Biology; Plant Sciences

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Citations

  • Machemer-Noonan, K. M. (2014). Interplay between unusual MYB transcription factors and their role in cell size regulation in plants [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397482170

    APA Style (7th edition)

  • Machemer-Noonan, Katja. Interplay between unusual MYB transcription factors and their role in cell size regulation in plants. 2014. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1397482170.

    MLA Style (8th edition)

  • Machemer-Noonan, Katja. "Interplay between unusual MYB transcription factors and their role in cell size regulation in plants." Doctoral dissertation, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397482170

    Chicago Manual of Style (17th edition)

osu1397482170
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This open access ETD is published by The Ohio State University and OhioLINK.