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John Grayson Masters Thesis.pdf (2.65 MB)

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Improvement of Yellow Perch Larvae Culture via Live Food Enrichment with Polyunsaturated Fatty Acids

Grayson, John David
http://rave.ohiolink.edu/etdc/view?acc_num=osu1417720966

Abstract Details

2014, Master of Science, Ohio State University, Environment and Natural Resources.
Limited experience with live food regimes and fragmentary knowledge of nutritional requirements have been inhibitors for the indoor-intensive production of Yellow Perch Perca flavescens larvae. Live food enrichment with polyunsaturated fatty acids (PUFA) is a proven means of increasing the growth and survival of larval fish, but no studies to date have tested this method on Yellow Perch. This thesis consists of two live food enrichment experiments carried out in May/June of 2013 and 2014, as well as lipid analysis of live food and fish samples from both years. The 2013 study examined the effect of live food enrichment with docosahexaenoic acid (DHA; C22:6[n-3]) and arachidonic acid (ARA; C20:4[n-6]) on the growth, survival, and swim bladder inflation of larval Yellow Perch. The 2014 experiment was similar in design, but compared PUFA enrichments in ethyl ester (EE) and triglyceride (TG) forms. Both experiments were conducted in two phases. The first phase was carried out in a recirculating system with nine 50 L conical tanks, initially stocked at 50-70 larvae/L. Live rotifers Brachionus plicatilis were provided to larvae for the first two days of exogenous feeding, before transitioning to Artemia franciscana nauplii for the remaining eight days of this phase. The second phase was carried out in nine 60 L cylindrical flow-through tanks, initially stocked with 10 larvae/L. During this phase, fish were fed Artemia nauplii for 3 days, then gradually transitioned to a formulated starter diet (Otohime A®) over a 7 day period. At the end of the first phase the ARA and DHA enriched groups had significantly (p=0.05) improved swim bladder inflation rates when compared to the control group. For the second phase, enriched groups had significantly larger mean weights and growth rates than the control. The EE-TG experiment was similar in design to the DHA-ARA experiment, except that the second phase was concluded after seven days of feeding. At the end of the first phase, the EE group had a significantly improved average weight and growth rate than the TG group. No significant trends were seen in the second phase. Following enrichment experiments, the fatty acid composition of live feeds and experimental fish were analyzed using the gas chromatography method. Fatty acid composition of zooplankton was heavily influenced by enrichments, and composition of larvae/juveniles generally reflects that of their live prey. Arachidonic acid was assimilated poorly in ARA enriched Artemia, but DHA was found in abundance. Also, rotifers tended to assimilate PUFA better in EE form, while Artemia achieved higher PUFA contents with TG enrichments. The data support that PUFA enrichment of live food can be utilized to increase the success of Yellow Perch culture by increasing growth and swim bladder inflation rates during the critical period of larval development.
Konrad Dabrowski, Dr. (Advisor)
Suzanne Gray, Dr. (Committee Member)
Robert Gates, Dr. (Committee Member)
Ana Hill, Dr. (Committee Member)
73 p.
Animal Sciences; Aquaculture; Aquatic Sciences; Nutrition
yellow perch; polyunsaturated fatty acid; live food enrichment; rotifer; Artemia; docosahexaenoic acid; arachidonic acid; ethyl ester; triglyceride; aquaculture

Recommended Citations

Citations

  • Grayson, J. D. (2014). Improvement of Yellow Perch Larvae Culture via Live Food Enrichment with Polyunsaturated Fatty Acids [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1417720966

    APA Style (7th edition)

  • Grayson, John. Improvement of Yellow Perch Larvae Culture via Live Food Enrichment with Polyunsaturated Fatty Acids. 2014. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1417720966.

    MLA Style (8th edition)

  • Grayson, John. "Improvement of Yellow Perch Larvae Culture via Live Food Enrichment with Polyunsaturated Fatty Acids." Master's thesis, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1417720966

    Chicago Manual of Style (17th edition)

osu1417720966
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This open access ETD is published by The Ohio State University and OhioLINK.