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Mengmeng Xu dissertation.pdf (3.02 MB)

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Comparative genomic analysis and metabolic engineering of Clostridium acetobutylicum for enhanced n-butanol tolerance and production

Xu, Mengmeng
http://rave.ohiolink.edu/etdc/view?acc_num=osu1417777228

Abstract Details

2014, Doctor of Philosophy, Ohio State University, Biochemistry Program, Ohio State.
Clostridium acetobutylicum JB200, a mutant strain of C. acetobutylicum ATCC 55025 obtained through strain evolution in a fibrous bed bioreactor, had high butanol tolerance, and produced up to ~21 g/L butanol from glucose in batch fermentation, an improvement of ~67% over the parental strain (~12.6 g/L). To explore the genetic differences between the three C. acetobutylicum strains JB200, ATCC 55025, and the type strain ATCC 824, the whole genomes of ATCC 55025 and JB200 were sequenced using high-throughput Illumina sequencing technology. Comparative genomic analysis performed for the three strains identified many variations that may contribute to the non-spore-forming and enhanced butanol-producing characteristics in both ATCC 55025 and JB200. In addition, the comparative analysis revealed only seven point mutations in the genome of JB200, which might contribute to the greatly improved butanol production and tolerance of JB200 compared to ATCC 55025. Among the seven mutations, a single base deletion in the coding region of the cac3319 gene causes a large portion (~75% of the original length) of truncation at the C-terminus of its encoding histidine kinase. The inactivation of cac3319 in ATCC 55025 indicated the important role of cac3319 in regulating butanol tolerance and production of C. acetobutylicum. This study provides a molecular-level understanding of non-sporulation, higher butanol tolerance and production in C. acetobutylicum mutants, revealing possible regulatory factors involved in butanol tolerance and production.
Shang-Tian Yang (Advisor)
Jeffrey Chalmers (Committee Member)
Andre Palmer (Committee Member)
Hua Wang (Committee Member)
210 p.
Biochemistry; Bioinformatics; Chemical Engineering
ABE fermentation; Clostridium acetobutylicum; comparative genomic analysis; solventogenesis; butanol tolerance; histidine kinase; gene knockout; metabolic engineering

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Citations

  • Xu, M. (2014). Comparative genomic analysis and metabolic engineering of Clostridium acetobutylicum for enhanced n-butanol tolerance and production [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1417777228

    APA Style (7th edition)

  • Xu, Mengmeng. Comparative genomic analysis and metabolic engineering of Clostridium acetobutylicum for enhanced n-butanol tolerance and production. 2014. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1417777228.

    MLA Style (8th edition)

  • Xu, Mengmeng. "Comparative genomic analysis and metabolic engineering of Clostridium acetobutylicum for enhanced n-butanol tolerance and production." Doctoral dissertation, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1417777228

    Chicago Manual of Style (17th edition)

osu1417777228
968
© 2014, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.