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Studies of Enhanced-Fluidity Liquids for Chiral Separations

Sun, Qian
http://rave.ohiolink.edu/etdc/view?acc_num=osu1420637683

Abstract Details

2000, Master of Science, Ohio State University, Chemistry.
In the field of chiral separations by liquid chromatography, many efforts have been made on the optimization of the mobile phases for improvement in chromatographic performance and chiral selectivity. In this study, the application of enhanced-fluidity liquids as mobile phases for chiral separations is investigated. Enhanced-fluidity liquids are mixtures of normal organic solvents and liquified gases such as CO2 and CHF3. They combine the advantages of both supercritical fluids (lower viscosity and higher diffusivity) and pure liquid solvents (high solvating power). Under normal-phase condition, the highest resolution for chiral enantiomer separation is always achieved under the enhanced-fluidity liquid chromatography (EFLC) condition compared to those obtained under both supercritical fluid chromatography (SFC) and high performance liquid chromatography (HPLC) conditions. Improved efficiency and shorter analysis time are also observed for most separations with CO2 proportion less than 60 mol%. The use of EFLC in reversed-phase separation is also demonstrated with methanol/H2O/CO2 and methanol/H2O/CHF3 mixtures. The improvement on resolution is significant. Lower pressure drop across the column is another advantage provided by EFLC separations. In order to improve chiral selectivity, a chiral selector, vancomycin, is applied as both stationary phase chiral selector and chiral mobile phase additive (CMPA). Thermodynamic study demonstrates the difference in separation process between this chiral stationary phase (CSP)-CMPA system and that where CSP is used alone. Possible reasons for this observation are discussed. Significantly increased chiral discrimination is observed for this CSP-CMPA system. The highest resolution is observed when EFLC technique is used in this CSP-CMPA system with CHF3 as the fluidity enhancing solvent.
Susan V. Oleisk (Advisor)
Prabir K. Dutta (Committee Member)
55 p.
Chemistry

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Citations

  • Sun, Q. (2000). Studies of Enhanced-Fluidity Liquids for Chiral Separations [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1420637683

    APA Style (7th edition)

  • Sun, Qian. Studies of Enhanced-Fluidity Liquids for Chiral Separations. 2000. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1420637683.

    MLA Style (8th edition)

  • Sun, Qian. "Studies of Enhanced-Fluidity Liquids for Chiral Separations." Master's thesis, Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu1420637683

    Chicago Manual of Style (17th edition)

osu1420637683
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© 2000, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.