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Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond

Teeling-Smith, Richelle Marie
http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811

Abstract Details

2015, Doctor of Philosophy, Ohio State University, Physics.
Electron paramagnetic resonance (EPR) is an established and powerful tool for studying atomic-scale biomolecular structure and dynamics. Yet it requires a homogeneous sample size of approximately 10^15 spin-labeled biomolecules. In contrast, single molecule measurements provide improved insights into heterogeneous behaviors that can be masked by ensemble measurements and are often essential for illuminating the molecular mechanisms behind the function of a biomolecule. In this dissertation, I report EPR measurements of a single labeled biomolecule demonstrating the merging of these two powerful techniques. We have selectively labeled individual double-stranded DNA molecules with nanodiamonds containing nitrogen-vacancy (NV) centers, and optically detected the paramagnetic resonance of the NV nanodiamond probe. Analysis of the spectrum reveals that the characteristic time scale for reorientation of the labeled molecule relative to the applied magnetic field is slow compared to the spin relaxation time of the nanodiamond probe. This demonstration of EPR spectroscopic determination of the dynamics of an individual labeled biomolecular construct provides the foundation for single molecule magnetic resonance studies of complex biomolecular systems. In addition to this single-molecule EPR study, I report on the use of the NV center in diamond as a probe for in-vivo magnetometry, in-vivo fluorescence imaging and temperature sensing, and a tool for the measure of spin resonances in neighboring systems. The NV center in diamond has proven to be powerful tool for sensing and imaging in various systems, with a broad array of undeveloped or underdeveloped applications. The studies included in this dissertation provide a brief overview of a select few experiments that explore these capabilities.
P. Chris Hammel (Advisor)
Ezekiel Johnston-Halperin (Committee Member)
Michael G. Poirier (Committee Member)
Ralf Bundschuh (Committee Member)
201 p.
Biochemistry; Biophysics; Condensed Matter Physics; Molecular Physics; Nanoscience; Nanotechnology; Optics; Physics; Scientific Imaging
NV diamond; Nitrogen-Vacancy Diamond; EPR; Electron Paramagnetic Resonance; DNA; DNA dynamics; confocal microscopy; single molecule spectroscopy; single-molecule measurements; biophysics; biomolecular dynamics; ODMR; Optically detected magnetic resonance

Recommended Citations

Citations

  • Teeling-Smith, R. M. (2015). Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811

    APA Style (7th edition)

  • Teeling-Smith, Richelle. Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond. 2015. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811.

    MLA Style (8th edition)

  • Teeling-Smith, Richelle. "Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond." Doctoral dissertation, Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811

    Chicago Manual of Style (17th edition)

osu1424779811
531
© 2015, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.