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Stephanie Diamond - Thesis.pdf (823 KB)

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Determination of the Effect of Raspberry Ketone on Markers of Obesity in High-fat Fed C57BL/6 Male Mice

Diamond, Stephanie A
http://rave.ohiolink.edu/etdc/view?acc_num=osu1429626524

Abstract Details

2015, Master of Science, Ohio State University, Food Science and Technology.
Raspberry Ketone (RK) is a naturally occurring aroma compound in raspberries. Its chemical structure is similar to capsaicin and synephrine, which studies have shown to have weight loss effects. Preliminary studies indicate that RK may promote secretion of adiponectin, a fat regulating hormone, from adipocytes in cell culture and it has anti-obesity effects when fed to mice. Despite minimal published literature supporting RK’s utility as a fat and body-weight regulating compound, it is sold as a weight-loss supplement. These supplements are often marketed as adiponectin stimulating compounds, but there is no published study of RK’s effect on adiponectin in-vivo. Further, the mouse studies that have investigated RK’s biological action did not validate the presence of RK in the formulated diet. The goal of this study is two-fold: to develop an HPLC method for extraction and quantification of RK in high-fat mouse food, and to investigate the hypothesis that RK attenuates weight gain in mice fed a high fat diet (HFD). A method for extracting RK from high-fat mouse diet was optimized by solubilizing with ethyl acetate, drying and reconstituting with equal parts methanol and hexanes. It was then analyzed using HPLC and an isocratic method equipped with a C18 column. It was determined that RK is stable to processing, but between 13 and 50% loss of compound occurred. C56BL/6 mice were fed a HFD for 2 weeks then randomized by body weight to four groups: n = 10 HFD + 0% RK (control), n = 10 0.25% RK (LRK) or n = 9 1.74% RK (HRK) and a group n = 9 pair-fed control diet to the ad-libitum intake of the LRK group. Mice were fed experimental diets for 5 weeks. Food intake and body weight were measured daily. Adiponectin in blood plasma was measured by immunoassay at week 0 (baseline), week 2 and week 5. No effect of RK on plasma adiponectin levels was observed. Control group food intake was significantly higher than RK-fed mice (P = 0.0014), and there was a strong interaction between body weight and food intake (P < 0.0001) suggesting that body and tissue weight depression in RK-fed mice is due to lower energy intake, not the biological effect of RK. RK-fed mice showed attenuated weight gain compared to control mice in a dose-dependent manner. Pair-fed mice showed nearly identical weight gain to the LRK group. Epididymal fat depot weight was 22.5% smaller in HRK mice than control and inguinal fat depot weight was 41.9% (p = 0.013) lower in HRK mice. It is speculated that RK diet exhibited a weight-attenuating effect because of the reduced energy intake of mice fed RK-diet compared to control rather than a biological mechanism invoked by RK. RK does not appear to have an effect on plasma levels of adiponectin in the time-frame studied. This research is important for informing RK supplement consumers and manufacturers of its efficacy.
Yael Vodovotz (Advisor)
Martha Belury (Committee Member)
Christopher Simons (Committee Member)
84 p.
Food Science; Nutrition
Raspberry Ketone; Obesity; Adiponectin; Mice; HPLC

Recommended Citations

Citations

  • Diamond, S. A. (2015). Determination of the Effect of Raspberry Ketone on Markers of Obesity in High-fat Fed C57BL/6 Male Mice [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429626524

    APA Style (7th edition)

  • Diamond, Stephanie. Determination of the Effect of Raspberry Ketone on Markers of Obesity in High-fat Fed C57BL/6 Male Mice. 2015. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1429626524.

    MLA Style (8th edition)

  • Diamond, Stephanie. "Determination of the Effect of Raspberry Ketone on Markers of Obesity in High-fat Fed C57BL/6 Male Mice." Master's thesis, Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429626524

    Chicago Manual of Style (17th edition)

osu1429626524
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This open access ETD is published by The Ohio State University and OhioLINK.