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Kristen Datte - Thesis 2015.pdf (2.06 MB)

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Examination of the canine sublingual and gastric microcirculation in health and gastric dilatation volvulus

Datte, Kristen
http://rave.ohiolink.edu/etdc/view?acc_num=osu1429738406

Abstract Details

2015, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
The microcirculation consists of arterioles, capillaries, and venules measuring <200 µm and are responsible for delivery of nutrients, such as oxygen and glucose, to tissues and removal of waste products, such as carbon dioxide and lactate. In health, the flow within these vessels is maintained locally through a process called autoregulation. However, when diseases develop, this autoregulation may be lost. Commonly monitored macrohemodynamics, such as heart rate and blood pressure, have been evaluated and in general have not been found to correlate well with microvascular perfusion. Therefore, direct examination of the microcirculation has become an area of exploration in both the research and clinical settings. One such tool is sidestream dark field microscopy (SDM), which has utilized in both human and veterinary medicine. The microcirculation has been studied in a variety of tissue beds and in several disease conditions. In humans, several studies have found a correlation between the sublingual tissue and the gastric tissue, making the sublingual site a feasible surrogate for assessing gastrointestinal perfusion. However, few studies have been performed in veterinary medicine. No studies have evaluated microcirculation in gastric dilatation volvulus, even though this disease is relatively prevalent in the veterinary population. These studies were designed to help explore this area further. The first study performed by our group evaluated normal anaesthetized dogs receiving a prophylactic gastropexy. Using SDM, we evaluated the sublingual mucosal and gastric serosal microcirculation and concurrent systemic variables. Microcirculatory parameters were compared and evaluated for correlation between the tissue beds. Additionally, we evaluated for correlation between the microcirculation variables and systemic variables. The second study evaluated microcirculatory and systemic variables in patients with naturally occurring GDV, at several time points. Using SDM, we evaluated the sublingual mucosal and gastric serosal microcirculation at induction, prederotation, postderotation and on recovery. The microcirculation was evaluated for the number of vessels present and the perfusion of these vessels, including how many are perfused and the quality of the blood flow. Each of these features was then compared to previously established control dog variables and between the time points. The systemic variables were also compared to the microcirculation variables for evaluation of correlation. These studies successfully demonstrated the ability to perform intraoperative assessment of gastric and sublingual microcirculation. However, there did not appear to be significant correlation between the tissue beds in either patient population, making the sublingual tissue a poor surrogate for gastric tissue. Although a few correlations were found between the microvascular parameters and systemic variables, these are unlikely to be clinically significant. Finally, when compared to the microvascular variables of normal dogs, those dogs with naturally occurring GDV had altered variables at all time points. Furthermore, although gastric derotation resulted in significant improvement in gastric microcirculation, these values failed to return to those seen in the control population. Overall, we established the utility of SDM technology for evaluation of canine sublingual and gastric tissues. These results may be useful for future studies of normal canine abdominal organs as well as in disease states.
Edward Cooper (Advisor)
Monnig Andrea (Committee Member)
Ham Kathleen (Committee Member)
152 p.
Veterinary Services

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Citations

  • Datte, K. (2015). Examination of the canine sublingual and gastric microcirculation in health and gastric dilatation volvulus [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429738406

    APA Style (7th edition)

  • Datte, Kristen. Examination of the canine sublingual and gastric microcirculation in health and gastric dilatation volvulus. 2015. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1429738406.

    MLA Style (8th edition)

  • Datte, Kristen. "Examination of the canine sublingual and gastric microcirculation in health and gastric dilatation volvulus." Master's thesis, Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429738406

    Chicago Manual of Style (17th edition)

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