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The roles of OVATE and other elongation genes in regulating proximal-distal patterning of tomato fruit

Wu, Shan
http://rave.ohiolink.edu/etdc/view?acc_num=osu1437586702

Abstract Details

2015, Doctor of Philosophy, Ohio State University, Horticulture and Crop Science.
Domestication of tomato (Solanum lycopersicum) has resulted in a variety of fruit shapes from flat to very elongated. The elongated shape is a common feature that distinguishes many cultivated tomatoes from the undomesticated types, and it is mainly controlled by three loci, ovate, sun and fs8.1. We performed detailed morphological analyses of the reproductive and vegetative organs, which demonstrated that ovate, sun and fs8.1 regulate unique aspects of ovary and fruit elongation and in different temporal manners. The synergistic interaction between sun and ovate or fs8.1 suggested that the pathways involving SUN, OVATE and the gene(s) underlying fs8.1 may converge at a common node. We also conducted a transcriptome comparison analysis between the triple mutant sun/ovate/fs8.1 and wild type by RNA-seq using reproductive meristems and young flower buds. This study revealed changes in the transcription profile possibly caused by the combined effects of the three mutations. To gain insights into the role of OVATE in regulating fruit shape, we searched for its genetic and protein interactors. A synergistic interaction was found between ovate and suppressor of ovate 1 (sov1) in controlling ovary and fruit elongation. Tomato OVATE family protein 20 (SlOFP20) was identified as a candidate gene underlying the sov1 locus. Yeast two-hybrid (Y2H) experiments showed that OVATE interacted with Tonneau1 Recruiting Motif (TRM) proteins, which are a part of a protein complex regulating the formation of preprophase band and organization of cortical microtubule (MT) array. Transient co-expression of OVATE or SlOFP20 with putative MT-associated SlTRMs in N. benthamiana resulted in relocalizations of SlOFPs and SlTRMs. Our findings have shed new light on the roles of OFPs in proximal-distal fruit patterning and provided insights into fundamental aspects of plant organ growth.
Esther van der Knaap (Advisor)
Jang Jyan-chyun (Committee Member)
Michelle Jones (Committee Member)
Tea Meulia (Committee Member)
Eric Stockinger (Committee Member)
164 p.
Horticulture; Plant Biology
Tomato; Near Isogenic lines; Fruit shape; OVATE

Recommended Citations

Citations

  • Wu, S. (2015). The roles of OVATE and other elongation genes in regulating proximal-distal patterning of tomato fruit [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437586702

    APA Style (7th edition)

  • Wu, Shan. The roles of OVATE and other elongation genes in regulating proximal-distal patterning of tomato fruit. 2015. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1437586702.

    MLA Style (8th edition)

  • Wu, Shan. "The roles of OVATE and other elongation genes in regulating proximal-distal patterning of tomato fruit." Doctoral dissertation, Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437586702

    Chicago Manual of Style (17th edition)

osu1437586702
2,032
© 2015, some rights reserved.Creative Commons License The roles of OVATE and other elongation genes in regulating proximal-distal patterning of tomato fruit by Shan Wu is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.