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Electrochemical Characterization of ex vivo Human Hepatic Tissues Containing Colorectal Metastases and Quantification of Spatial Error in Electrical Impedance Mapping of Soft Tissues

Karnes, Michael
http://rave.ohiolink.edu/etdc/view?acc_num=osu1450118548

Abstract Details

2016, Master of Science, Ohio State University, Mechanical Engineering.
The American Cancer Society predicts that 49,700 Americans will die in the year 2015 because of colorectal cancer (CRC), making it the third most fatal cancer. Resection remains the most curative treatment for hepatic CRC metastases. It is the only treatment capable of providing patients with five year survival rates of over 50%. There are no commercially available intraoperative instruments capable of quantitatively determining the interface location between cancerous tissue and healthy hepatic tissue, leaving surgeons reliant upon palpation and visual cues when deciding which tissues should be removed. This thesis characterizes human ex vivo hepatic tissues containing CRC metastases and quantifies the spatial error of the impedance mapping technique in determining tissue interface locations using porcine-bovine muscular tissue phantoms, providing insight towards the feasibility of implementing an impedance mapping device for margin determination in the resection of CRC metastases. In total, the impedances of 183 unique locations were measured in ex vivo hepatic tissues containing CRC metastases from 100 Hz to 1 MHz, resulting in the largest set of published impedance data on these types of tissues to date. Using the Tukey-Kramer comparison method with 95% family confidence intervals, normal hepatic tissues were found to be significantly different than the CRC metastases in their impedance modulus, phase, conductivity and relative permittivity at certain frequencies. The phase at 25 kHz was found to provide the greatest difference in normal and tumor tissue response. Porcine-bovine muscular tissue phantoms were used to quantify the spatial accuracy of impedance mapping in predicting the interface locations in soft tissues, using a four electrode linear impedance probe with a 1.5 mm pitch and 0.5 cm step size between measurements. The spatial errors of the interface locations predicted by impedance mapping and the true interface locations were quantified in one dimension with three image reconstruction techniques: linear interpolation, linear regression, and logarithmic regression. The maximum probable error with 95% confidence for each image reconstruction technique was calculated using a four sigma interval of the calculated mean errors and standard deviations. Implementing the optimized thresholds, linear regression had lowest total mean maximum error at 0.256 cm followed by interpolation at 0.298 cm, and then by logarithmic regression at 1.942 cm. The degree of difference between CRC metastases and the surrounding hepatic tissue along with interface predictions with maximum errors of less than 0.3 cm show a potential for the application of impedance mapping for the intraoperative identification of tumor interface locations.
Shaurya Prakash, Dr. (Advisor)
Vishwanath Subramaniam, Dr. (Committee Member)
376 p.
Mechanical Engineering
Electrochemical Impedance Spectroscopy, Bioimpedance, Hepatic Colorectal Metastases, Impedance Imaging

Recommended Citations

Citations

  • Karnes, M. (2016). Electrochemical Characterization of ex vivo Human Hepatic Tissues Containing Colorectal Metastases and Quantification of Spatial Error in Electrical Impedance Mapping of Soft Tissues [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1450118548

    APA Style (7th edition)

  • Karnes, Michael. Electrochemical Characterization of ex vivo Human Hepatic Tissues Containing Colorectal Metastases and Quantification of Spatial Error in Electrical Impedance Mapping of Soft Tissues. 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1450118548.

    MLA Style (8th edition)

  • Karnes, Michael. "Electrochemical Characterization of ex vivo Human Hepatic Tissues Containing Colorectal Metastases and Quantification of Spatial Error in Electrical Impedance Mapping of Soft Tissues." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1450118548

    Chicago Manual of Style (17th edition)

osu1450118548
692
© 2016, some rights reserved.Creative Commons License Electrochemical Characterization of ex vivo Human Hepatic Tissues Containing Colorectal Metastases and Quantification of Spatial Error in Electrical Impedance Mapping of Soft Tissues by Michael Karnes is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.