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Wei Xue thesis 160422.pdf (5.01 MB)

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Measurements of Cellular Intrinsic Magnetism with Cell Tracking Velocimetry and Separation with Magnetic Deposition Microscopy

Xue, Wei, xue
http://orcid.org/0000-0003-1227-0334
http://rave.ohiolink.edu/etdc/view?acc_num=osu1461231847

Abstract Details

2016, Doctor of Philosophy, Ohio State University, Chemical Engineering.
Magnetic cell separation has been widely used in biotechnology because of its advantages in relatively easy of us, high sensitivity and selectivity. Though most magnetic separation is based on the immuno-labeling with magnetic particles, the recent remarkable increase on the strength of permanent magnets has enables label-less magnetic separation, which has its unique advantages when applicable. To better design and understand such magnetic separation process dependent on the intrinsic magnetism of cells, the technology with high sensitivity of characterizing such weakly paramagnetic cells is critical, yet rarely reported. The detection of paramagnetic levels of magnetic moment of cells enables the analysis of iron containing proteins such as ferritin, which is also of great interest because of their abnormal expression is closely related to cancer. In this dissertation, a magnetophoretic analysis instrument, cell tracking velocimetry, (CTV) was reviewed, upgraded, and applied to different intrinsic magnetic cells, and its sensitivity and accuracy was discussed in details. The recent innovations of CTV, which were used through the whole dissertation including internal control method, elevated magnetic energy density gradient and fluorescent options were elaborated. Then CTV was compared with a more commercially available magnetometer superconducting quantum interference-magnetic properties measurement system (SQUID-MPMS) by measuring 3 different forms of red blood cells (RBCs). The accuracy of the CTV is on par with the SQUID-MPMS and CTV is advantageous for its remarkable sensitivity, low sample size required, large quantity of results, and the ease of operation for biological cell samples. CTV were applied on different types of cells to analyze their weak magnetism. With the established superiority of CTV, the magnetic properties of HeLa cells with various iron supplementations were evaluated as a cancer iron metabolism model. The elevated magnetic susceptibility and successful deposition of HeLa cells on the magnetic deposition microscopy (MDM) demonstrated the potential of analysis and separation of circulating tumor cells in the peripheral blood. Also the HeLa proliferation was evaluated to discuss the role of iron in terms of HeLa growth. Also, with CTV we were able to select 3 strains of genetic modified green algae Auxenochlorella protothecoides (A.p.) for potential magnetic separation of bio-fuel producing algae based on their superior magnetic susceptibility. The iron content, the expression of iron related gene and the feasibility of magnetic separation were also confirmed. In terms of magnetic separation, an open fringing field magnetic separation device, magnetic deposition microscopy, (MDM), was elaborated. The strong fringing field gradient allows the deposition of weakly magnetic cells onto a Mylar slide for the further analysis. The deposition could be predicted with simulation software, which closely corresponded with the experiment result of Bacillus spores and methemoglobin red blood cells. The magnetic field flow fractionation (FFF) introduced as an analytical tool for magnetic particles. By manipulating the electro-magnetic field, a profile of the capture rate against the magnetic field and flow rate and of a magnetic nano-particle was generated, which could provide guidance in the magnetic separation process development.
Jeffrey Chalmers (Advisor)
David Wood (Committee Member)
Andre Palmer (Committee Member)
Timothy Plageman (Committee Member)
230 p.
Chemical Engineering
CTV; MDM; magnetic property analysis; magnetic separation

Recommended Citations

Citations

  • Xue, xue, W. (2016). Measurements of Cellular Intrinsic Magnetism with Cell Tracking Velocimetry and Separation with Magnetic Deposition Microscopy [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461231847

    APA Style (7th edition)

  • Xue, xue, Wei. Measurements of Cellular Intrinsic Magnetism with Cell Tracking Velocimetry and Separation with Magnetic Deposition Microscopy. 2016. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1461231847.

    MLA Style (8th edition)

  • Xue, xue, Wei. "Measurements of Cellular Intrinsic Magnetism with Cell Tracking Velocimetry and Separation with Magnetic Deposition Microscopy." Doctoral dissertation, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461231847

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.