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Protection of Washed and Pasteurized Shell Eggs against Fungal Growth by Application of Natamycin-Containing Shellac Coating

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2016, Master of Science, Ohio State University, Food Science and Technology.
The purpose of this study was to evaluate the antifungal efficacy of the natamycin- shellac coating on commercially washed eggs and pasteurized eggs. Eggs were prepared into three groups: eggs that received no coating (control), eggs that were coated with shellac only (treatment 1), and eggs that were coated with shellac containing 400 µg/ml of natamycin (treatment 2). Concentration of natamycin suitable for application on shell eggs was determined previously. Egg groups were inoculated with mold spores and stored at 25°C for 18 days, and mold growth were monitored by enumeration on potato dextrose agar (PDA). Molds tested in this study belong to three genera: Mucor, Cladosporium and Penicillium. The experiment was repeated three times and results were analyzed statistically. Treatments that permitted mold growth provided data that allowed the construction of growth curves. These growth curves were fitted with logistic model, and growth kinetic parameters (maximum population (A), maximum specific growth rate (µmax), and lag time were estimated and compared. According to parameters comparison between control and treatment 1, shellac coating did inhibit fungal growth on washed egg, but it exhibited antifungal effectiveness against Mucor and Penicillium on pasteurized eggs. Presence of natamycin in shellac coating significantly inhibited fungal contamination on both commercially washed eggs and pasteurized eggs. This study illustrated that shellac coating can be used as a carrier for natamycin to create an effective protection against mold contaminations of eggs, hence the antifungal agent can potentially extend the shelf-life of treated eggs and minimize economic lose.
Ahmed Yousef (Advisor)
Dennis Heldman (Committee Member)
Luis Rodriguez-Saona (Committee Member)
154 p.

Recommended Citations

Citations

  • Song, Y. (2016). Protection of Washed and Pasteurized Shell Eggs against Fungal Growth by Application of Natamycin-Containing Shellac Coating [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461274469

    APA Style (7th edition)

  • Song, Yang. Protection of Washed and Pasteurized Shell Eggs against Fungal Growth by Application of Natamycin-Containing Shellac Coating. 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1461274469.

    MLA Style (8th edition)

  • Song, Yang. "Protection of Washed and Pasteurized Shell Eggs against Fungal Growth by Application of Natamycin-Containing Shellac Coating." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461274469

    Chicago Manual of Style (17th edition)