Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

The effect of hypothermia on influx of mononuclear cells in the digital lamellae of horses with oligofructose-induced laminitis

Godman, Jennifer Delgado, BVMS, MRCVS

Abstract Details

2016, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
Sepsis-related laminitis (SRL) is a common complication in the septic/endotoxemic critically-ill equine patient, in which lamellar injury and failure commonly lead to crippling distal displacement of the distal phalanx. Similar to organ injury in human sepsis, lamellar injury in SRL has been associated with inflammatory events, including the influx of leukocytes into the lamellar tissue and markedly increased expression of a wide array of inflammatory mediators at the onset of Obel grade 1 (OG1) laminitis. The only treatment reported both clinically and experimentally to protect the lamellae in SRL, local hypothermia, has been demonstrated to effectively inhibit lamellar expression of multiple inflammatory mediators when initiated at the time of administration of a carbohydrate overload in experimental models of SRL. However, the effect of hypothermia on leukocyte influx into affected tissue has not been assessed. We hypothesized that hypothermia inhibits leukocyte emigration into the digital lamellae in SRL. Immunohistochemical staining using leukocyte markers MAC387 (marker of neutrophils, activated monocytes) and CD163 (monocyte/macrophage-specific marker) was performed on archived lamellar tissue samples from an experimental model of SRL in which one forelimb was maintained at ambient temperature (AMB) and one forelimb was immersed in ice water (ICE) immediately following enteral oligofructose administration (10g/kg, n=14 horses). Lamellae were harvested at 24 hours post-oligofructose administration (DEV, n=7) or at the onset of OG1 laminitis (OG1, n=7). Both MAC387-positive (+) and CD163-positive (+) cells were counted by a single blinded investigator on images [n=10 (20x fields/digit for MAC387 and 40x fields/digit for CD163)] obtained using Aperio microscopy imaging analysis software (Leica Biosystems Inc. Buffalo Grove, IL USA). Data were assessed for normality and analyzed with a paired t-test and one-way ANOVA with significance set at p<0.05. MAC387(+) cells were present in low numbers in the lamellar tissue and were decreased in the hypothermic limbs (vs. AMB limbs, p<0.05) in the OG1 group; no change in CD163(+) cell numbers was noted across the conditions of the model. This study demonstrated that hypothermia of the distal limbs instituted early in the disease process in the horse at risk of SRL significantly attenuates the increase of MAC387(+) leukocytes in the digital lamellae, but has minimal effect on increases in lamellar concentrations of the major leukocyte cell type present in that tissue, CD163(+) mononuclear cells.
James Belknap, DVM PhD (Advisor)
Teresa Burns, DVM PhD (Committee Member)
Margaret Mudge, VMD (Committee Member)
Eric Schroeder, DVM MS (Committee Member)
58 p.

Recommended Citations

Citations

  • Godman, BVMS, MRCVS, J. D. (2016). The effect of hypothermia on influx of mononuclear cells in the digital lamellae of horses with oligofructose-induced laminitis [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1462843751

    APA Style (7th edition)

  • Godman, BVMS, MRCVS, Jennifer. The effect of hypothermia on influx of mononuclear cells in the digital lamellae of horses with oligofructose-induced laminitis. 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1462843751.

    MLA Style (8th edition)

  • Godman, BVMS, MRCVS, Jennifer. "The effect of hypothermia on influx of mononuclear cells in the digital lamellae of horses with oligofructose-induced laminitis." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1462843751

    Chicago Manual of Style (17th edition)