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The Effects of CYC-B Introgressions on Cherry Tomato Fruit Quality

Sari, Eka
http://rave.ohiolink.edu/etdc/view?acc_num=osu1469645306

Abstract Details

2016, Master of Science, Ohio State University, Horticulture and Crop Science.
Carotenoid compounds are known to have benefits for human health. ß-carotene is a carotenoid compound from plants that supplies the most reliable source of pro-vitamin A in the human diet. Natural variation for ß-carotene levels exists in tomato making it a promising target for genetic improvement through introgression. ß-carotene production in tomato is genetically controlled by the B locus. The structural gene at B (CYC-B) encodes the fruit specific lycopene ß-cyclase. Increased CYC-B expression is responsible for elevating ß-carotene levels in fruit. Nucleotide variation in the CYC-B promoter was identified as the likely cause of alterations in ß-carotene concentrations. Polymorphisms in this region can be used as markers to select for tomato with high ß-carotene levels. In the work described in this thesis, alleles of B originating from several wild species were introgressed into a grape-cherry tomato, Tainan (PI 647556). To facilitate this work, 1600 bp of the Tainan CYC-B promoter was sequenced. Cluster analysis of the CYC-B promoter with 109 other tomato accessions extracted from publicly available genome sequences revealed a close relationship between Tainan and wild cherry tomatoes, and distinct from the allele frequently present in cultivated tomatoes. Sequence alignment revealed several polymorphisms that were used to establish markers for B allele selection in the Tainan genetic background. The markers were employed for BC1 and BC2 and selfed progeny obtained from the selections. In the second-phase of selection, marker-assisted backcrossing (MAB) was used to accelerate the breeding progress by improving recurrent parent genome recovery during two backcross generations. Background genotyping was performed on the BC1 B-containing plants aiming to select Tainan alleles for loci controlling fruit weight, shape, and size located on chromosome 2 and 11. In BC2 families, B-selections were screened with 24 markers distributed across the genome. The application of background genotyping facilitated the identification and selection of BC2 individuals with the equivalent recurrent parent genome expected in BC3 to BC5 plants. Phenotypic evaluation of fruit quality traits were conducted on BC1S1 and BC2S1 populations with quantitative data collected for carotenoids, soluble solids, titratable acids, fruit size and shape, and sensory attributes as measured by trained panelists. Differences were detected depending on the source of the B allele. Progeny with the 97L97 B allele, derived from S. galapagense, had the highest ß-carotene and soluble solid levels with fruit size and shape being the most similar to the recurrent parent. Trained panelists consistently detected differences in flavor attributes that depended on the source of the B allele. The results helped me to determine quality attributes associated with B alleles derived from different sources.
David Francis, Dr (Advisor)
Joseph Scheerens, Dr (Committee Member)
Leah McHale, Dr (Committee Member)
146 p.
Horticulture

Recommended Citations

Citations

  • Sari, E. (2016). The Effects of CYC-B Introgressions on Cherry Tomato Fruit Quality [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1469645306

    APA Style (7th edition)

  • Sari, Eka. The Effects of CYC-B Introgressions on Cherry Tomato Fruit Quality . 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1469645306.

    MLA Style (8th edition)

  • Sari, Eka. "The Effects of CYC-B Introgressions on Cherry Tomato Fruit Quality ." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1469645306

    Chicago Manual of Style (17th edition)

osu1469645306
818
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