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Deciphering and Expending Clostridium formicoaceticum Metabolism Based on Whole Genome Sequencing

Bao, Teng
http://rave.ohiolink.edu/etdc/view?acc_num=osu1480631398782307

Abstract Details

2016, Master of Science, Ohio State University, Chemical Engineering.
Clostridium formicoaceticum is a Gram-negative homoacetogenic bacterium for acetic acid production and is also considered as a promising heterologous host for biofuel production. Here, its genome has been completely sequenced and consists of a 4.5-Mbp chromosome without containing any plasmid. The results of sequence analysis indicate that C. formicoaceticum is able to grow on several sugars and other organic substrates. In addition, it also exhibits a highly conserved Wood-Ljungdahl pathway gene cluster, which shows an identical arrangement as that in other autotrophic acetogens, such as Clostridium aceticum, Clostridium carboxidivorans, and Clostridium ljungdahlii. Differently from these homoacetogens, C. formicoaceticum contains a Na+-translocating ATPase and Rnf system for energy conversion. Such ATP generation system has also been sufficiently elucidated in Acetobacterium woodii. However, no growth defects were observed when C. formicoaceticum was grown on the heterotrophic and autotrophic medium under low sodium ion concentration, respectively. Otherwise, unlike Moorella thermoacetica and C. aceticum, neither completely cytochromes nor quinones synthesis genes are identified in the genome. Therefore, like C. ljungdahlii, C. formicoaceticum generates proton gradient via Rnf system and belongs to a H+-type homoacetogen. Moreover, ethanol formation may proceed by acetaldehyde dehydrogenases, alcohol dehydrogenases, and reversible aldehyde oxidoreductases under excessive reducing equivalent condition. Such information will be valuable in developing strategies for fermentation and metabolic engineering to produce bio-based chemicals and fuels in C. formicoaceticum.
Shang-Tian Yang (Advisor)
Jeffrey Chalmers (Committee Member)
62 p.
Chemical Engineering
Deciphering and Expending Clostridium formicoaceticum Metabolism, Whole Genome Sequencing

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Citations

  • Bao, T. (2016). Deciphering and Expending Clostridium formicoaceticum Metabolism Based on Whole Genome Sequencing [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1480631398782307

    APA Style (7th edition)

  • Bao, Teng. Deciphering and Expending Clostridium formicoaceticum Metabolism Based on Whole Genome Sequencing . 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1480631398782307.

    MLA Style (8th edition)

  • Bao, Teng. "Deciphering and Expending Clostridium formicoaceticum Metabolism Based on Whole Genome Sequencing ." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1480631398782307

    Chicago Manual of Style (17th edition)

osu1480631398782307
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© 2016, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.