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Enhancing Saugeye (Sander vitreus x S. canadensis) Production Through the Use of Assisted-Reproduction Technologies

Blawut, Bryan Joseph

Abstract Details

2017, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
The overall objective of this thesis was to increase the efficiency of saugeye (Sander vitreus x S. canadensis) production in the Ohio Department of Natural Resources hatchery system through the study of sauger (Sander canadensis) sperm. In the first experiment, we investigated the efficacy of sauger sperm cryopreservation in addition to determining the effects of base extender osmolality on sperm cryosurvival. Aliquots of sperm from ten male sauger were diluted with extenders with osmolalities of 350, 500 and 750 mOsm/kg (extender 350, 500 and 750, respectively) to a final concentration of 5.0 x 10 8 sperm/mL in extender containing 10% DMSO. Samples were placed at 3 cm above liquid nitrogen for 10 minutes, plunged into liquid nitrogen, and then thawed for 30 seconds at 21°C. Sperm parameters (total motility, progressive motility and velocity) were objectively assessed at different steps of the cryopreservation process. Viability was determined for thawed sperm. Cryoprotectant addition decreased sperm velocity in all extenders, but increased progressive motility in extender 350 and 500. Total motility was not affected by CPA addition in extender 350 and 500 but decreased in extender 750. All parameters measured, except progressive motility, were significantly affected by cryopreservation. Extender 500 produced the highest post-thaw progressive motility (32.20 ± 1.20%) and velocity (84.97 ± 5.32 µm/s) while both extender 350 and 500 displayed the highest total motility (65.30 ± 1.40 and 68.70 ± 2.00%) and viability (80.60 ± 1.50 and 78.80 ± 1.20%), respectively. Extender 750 produced the lowest post-thaw velocity (38.17 ±2.11 µm/s), viability (71.80 ± 1.20%), total (12.10 ± 1.60%) and progressive motility (1.60 ± 0.60%). The purpose of our second study was to decrease sauger sperm limitation during saugeye production in fish hatcheries. Towards this end, we investigated the effect of hCG administration on sperm production, the effect of a fertilization solution and sperm-to-egg ratios on egg fertilization rates and validated the use of a commercially available densimeter to determine sauger sperm cell concentration. We found that hCG increased sperm volume relative to controls (hCG 0.64 ± 0.19 mL/kg; control: 0.31 ± 0.05 mL/kg) but sperm cell concentration and total sperm production were unaffected. Fertilization rates similar among the three sperm-to-egg ratios tested (20,000/egg: 82.67 ± 3.39%, 50,000/egg: 91.33 ± 2.67% and 100,000/egg: 88.67 ± 3.44%) but were significantly decreased when an activation solution (16.00 ± 2.70%) compared to hatchery water (87.56 ± 2.04%). Lastly, we compared the use of a densimeter versus a hemocytometer and determined that a high correlation exists between the two methods used for calculation of sperm concentration (R2 = 96.37%). These results can be used by hatchery staff working with sauger and saugeye to more efficiently quantify and utilize sauger sperm for improved saugeye production efficiency. We conclude that the results of this thesis can be implemented in the state hatchery system to preserve high quality sauger sperm in the long term while also standardizing the use of fresh sperm in the short term with the goal of increasing saugeye production.
Marco Coutinho da Silva, DVM (Advisor)
Barbara Wolfe, DVM (Committee Member)
Stuart Ludsin, PhD (Committee Member)
102 p.

Recommended Citations

Citations

  • Blawut, B. J. (2017). Enhancing Saugeye (Sander vitreus x S. canadensis) Production Through the Use of Assisted-Reproduction Technologies [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492169462975574

    APA Style (7th edition)

  • Blawut, Bryan. Enhancing Saugeye (Sander vitreus x S. canadensis) Production Through the Use of Assisted-Reproduction Technologies. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1492169462975574.

    MLA Style (8th edition)

  • Blawut, Bryan. "Enhancing Saugeye (Sander vitreus x S. canadensis) Production Through the Use of Assisted-Reproduction Technologies." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492169462975574

    Chicago Manual of Style (17th edition)