Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Dissertation Julio Fatoretto Final.pdf (1.54 MB)

ETD Abstract Container

Abstract Header

Molecular Characterization of Fall Armyworm (Spodoptera frugiperda) Resistant to Vip3Aa20 Protein Expressed in Corn

Fatoretto, Julio Cesar
http://rave.ohiolink.edu/etdc/view?acc_num=osu1494233033064726

Abstract Details

2017, Doctor of Philosophy, Ohio State University, Translational Plant Sciences.
Transgenic plants containing genes from Bacillus thuringiensis have been used as an alternative to chemical insecticides for insect pest control. The vegetative insecticidal proteins (Vip) secreted during the vegetative growth phase of bacteria are considered a second generation of insecticidal proteins since they do not share any structural or sequence homology with previously used crystal proteins (Cry) as well as having a wide insecticidal spectrum. One of the target pests for this protein is the fall armyworm (FAW) (Spodoptera frugiperda), the most important corn pest in South America. Previously it has been controlled by insecticides and corn expressing Cry proteins, but has rapidly evolved resistance to many control practices and remains a top concern for sustainable biotechnology control efforts. Thus, resistance characterization involving mode of action and genetics of resistance can help with Insect Resistance Management strategies, and improve the durability of control. In this dissertation, using selected FAW population resistant to Vip3Aa20 Bt protein (Vip-R1and Vip-R2) we generated comparative proteomic and transcriptomic data among resistant and susceptible colonies. In the chapter 2, we bring FAW biology/ecology and Brazilian agriculture landscape data to support the high adaptive potential of this pest to genetically modified corn expressing Bt Cry proteins in Brazil. Proteomics studies in the chapter 3 revealed that neither Vip-R1 nor Vip-R2 showed difference between resistant and susceptible colonies either for Vip3Aa20 activation through proteolysis assay nor protein binding to the receptor. Transcriptomic sequencing and RNA-seq analysis in the chapter 4 showed strong evidence of ABC transporter genes associated with resistance as well as genes related to G-protein signaling pathway as downregulated. These results will be discussed in context of providing best management practices for managing FAW resistance to Vip, and extending the durability of Vip technology.
Andrew Michel, Dr (Advisor)
152 p.
Agriculture; Biochemistry; Entomology; Molecular Biology
Fall armyworm; Vip3A; Insect resistance management; Protein-receptor binding; Transcriptome; RNA-seq; ABC transporter

Recommended Citations

Citations

  • Fatoretto, J. C. (2017). Molecular Characterization of Fall Armyworm (Spodoptera frugiperda) Resistant to Vip3Aa20 Protein Expressed in Corn [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1494233033064726

    APA Style (7th edition)

  • Fatoretto, Julio. Molecular Characterization of Fall Armyworm (Spodoptera frugiperda) Resistant to Vip3Aa20 Protein Expressed in Corn. 2017. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1494233033064726.

    MLA Style (8th edition)

  • Fatoretto, Julio. "Molecular Characterization of Fall Armyworm (Spodoptera frugiperda) Resistant to Vip3Aa20 Protein Expressed in Corn." Doctoral dissertation, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1494233033064726

    Chicago Manual of Style (17th edition)

osu1494233033064726
635
© 2017, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.