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Magnetic Actuation of Biological Systems

Lauback, Stephanie Diane
http://orcid.org/0000-0002-0706-6200
http://rave.ohiolink.edu/etdc/view?acc_num=osu1494262695434601

Abstract Details

2017, Doctor of Philosophy, Ohio State University, Physics.
Central to the advancement of many biomedical and nanotechnology capabilities is the capacity to precisely control the motion of micro and nanostructures. These applications range from single molecule experiments to cell isolation and separation, to drug delivery and nanomachine manipulation. This dissertation focuses on actuation of biological micro- and nano-entities through the use of weak external magnetic fields, superparamagnetic beads, and ferromagnetic thin films. The magnetic platform presents an excellent method for actuation of biological systems due to its ability to directly control the motion of an array of micro and nanostructures in real-time with calibrated picoNewton forces. The energy landscape of two ferromagnetic thin film patterns (disks and zigzag wires) is experimentally explored and compared to corresponding theoretical models to quantify the applied forces and trajectories of superparamagnetic beads due to the magnetic traps. A magnetic method to directly actuate DNA nanomachines in real-time with nanometer resolution and sub-second response times using micromagnetic control was implemented through the use of stiff DNA micro-levers which bridged the large length scale mismatch between the micro-actuator and the nanomachine. Compared to current alternative methods which are limited in the actuation speeds and the number of reconfiguration states of DNA constructs, this magnetic approach enables fast actuation (~ milliseconds) and reconfigurable conformations achieved through a continuous range of finely tuned steps. The system was initially tested through actuation of the stiff arm tethered to the surface, and two prototype DNA nanomachines (rotor and hinge) were successfully actuated using the stiff mechanical lever. These results open new possibilities in the development of functional robotic systems at the molecular scale. In exploiting the use of DNA stiff levers, a new technique was also developed to investigate the emergence of the magnetization of individual superparamagnetic beads as a function of the applied field. Last, since proteins are frequently used for surface adhesion in assembling biomedical devices, preliminary tests were implemented to dynamically pattern proteins on a substrate using transformed E. coli that are magnetically labeled
Ratnasingham Sooryakumar (Advisor)
187 p.
Physics
micromagnetic actuation, DNA origami, hinge, rotor, lever arm, real-time, magnetization, permanent moment, anisotropic induced moment, easy axis MyOne Dynabeads, energy landscape, patterned magnetic thin films, E coli

Recommended Citations

Citations

  • Lauback, S. D. (2017). Magnetic Actuation of Biological Systems [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1494262695434601

    APA Style (7th edition)

  • Lauback, Stephanie. Magnetic Actuation of Biological Systems. 2017. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1494262695434601.

    MLA Style (8th edition)

  • Lauback, Stephanie. "Magnetic Actuation of Biological Systems." Doctoral dissertation, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1494262695434601

    Chicago Manual of Style (17th edition)

osu1494262695434601
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© 2017, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.