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Polyamines Mitigate Antibiotic Inhibition of A.actinomycetemcomitans Growth

Wattimena, Allan
http://rave.ohiolink.edu/etdc/view?acc_num=osu1498143446132264

Abstract Details

2017, Master of Science, Ohio State University, Dentistry.
Polyamines are ubiquitous polycationic molecules that are present in all prokaryotic and eukaryotic cells. They are the breakdown products of amino acids and are important modulators of cell growth, stress and cell proliferation. Polyamines are present in higher concentrations in the periodontal pocket and may affect antibiotic resistance of bacterial biofilms. The effect of polyamines was investigated with amoxicillin (AMX), azithromycin (AZM) and doxycycline (DOX) on the growth of Aggregatibacter actinomycetemcomitans (A.a.) Y4 strain. Bacteria were grown in brain heart infusion broth under the following conditions: 1) A.a. only, 2) A.a. + antibiotic, 3) A.a. + antibiotic + polyamine mix (1.4mM putrescine, 0.4mM spermidine, 0.4mM spermine). Growth curve analysis, MIC determination and metatranscriptomic analysis were carried out. The presence of exogenous polyamines produced a small, but significant increase in growth of A.a. Polyamines mitigated the inhibitory effect of AMX, AZM and DOX on A.a. growth. Metatranscriptomic analysis revealed differing transcriptomic profiles when comparing AMX and AZM in the presence of polyamines. Polyamines produced a transient mitigation of AMX inhibition, but did not have a significant effect on gene transcription. Many gene transcription changes were seen when polyamines were in the presence of AZM. Polyamines in a periodontal pocket may help protect A.a. and contribute to antimicrobial resistance.
John Walters (Advisor)
Purnima Kumar (Committee Member)
Shareef Dabdoub (Committee Member)
Sara Palmer (Committee Member)
84 p.
Dentistry

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Citations

  • Wattimena, A. (2017). Polyamines Mitigate Antibiotic Inhibition of A.actinomycetemcomitans Growth [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1498143446132264

    APA Style (7th edition)

  • Wattimena, Allan. Polyamines Mitigate Antibiotic Inhibition of A.actinomycetemcomitans Growth. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1498143446132264.

    MLA Style (8th edition)

  • Wattimena, Allan. "Polyamines Mitigate Antibiotic Inhibition of A.actinomycetemcomitans Growth." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1498143446132264

    Chicago Manual of Style (17th edition)

osu1498143446132264
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This open access ETD is published by The Ohio State University and OhioLINK.