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Characterization of Reperfusion Injury-Induced ROS in Striated Muscles

Chuang, Chia-Chen

Abstract Details

2017, Master of Science, Ohio State University, Biophysics.
Reactive oxygen species (ROS) are naturally generated within the biological system, and their levels are carefully controlled by the endogenous antioxidants. ROS are involved in mediating several physiological responses such as muscle contraction and immune defenses. To maintain regular muscular activity, ample amount of ROS is necessary, yet stresses and pathological assaults can induce excess ROS production, suggesting the complicated and hermetic roles of ROS. Striated muscles subjected to hypoxia or ischemia followed by reperfusion is susceptible to oxidative stress-related injury and subsequent muscle function decline. Therapeutics such as hypoxic preconditioning (HPC) and pharmacological intervention has been proposed to alleviate reperfusion-induced injuries. However, the exact mechanisms underlying these protective effects are not fully elucidated. In my first project, we examined HPC protection on the diaphragms of pulmonary TNF-alpha overexpressing (Tg+) mice (COPD-like model). We observed improved muscle fatigue resistance in HPC-treated diaphragm during reoxygention. This is likely attributed to ROS-initiated downstream activation of PI3K-Akt-ERK pathways as well as regulation of related mitochondrial channels. My second project investigated the functional cardioprotective properties of paeonol, an active herbal compound with antioxidant activity, using rat ischemia/reperfusion (I/R) model. Our result showed that pre-treatment of paeonol minimized I/R-associated no-reflow injuries, and may be considered as a potential preventive treatment for cardiac diseases or post-coronary revascularization in which no-reflow often occurs. Lastly, my third project identified the involvement of ROS in overstretching-induced skeletal muscle force decline.
Li Zuo (Advisor)
Hua Zhu (Committee Member)
113 p.

Recommended Citations

Citations

  • Chuang, C.-C. (2017). Characterization of Reperfusion Injury-Induced ROS in Striated Muscles [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1500479949278294

    APA Style (7th edition)

  • Chuang, Chia-Chen. Characterization of Reperfusion Injury-Induced ROS in Striated Muscles. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1500479949278294.

    MLA Style (8th edition)

  • Chuang, Chia-Chen. "Characterization of Reperfusion Injury-Induced ROS in Striated Muscles." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1500479949278294

    Chicago Manual of Style (17th edition)