Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


JK DEN Thesis Final.pdf (1.42 MB)

ETD Abstract Container

Abstract Header

Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis

Kim, Joshua B
http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724

Abstract Details

2017, Master of Science, Ohio State University, Nutrition Program, The Ohio State University.
Nonalcoholic steatohepatitis (NASH) increases hepatocellular carcinoma (HCC) risk by increasing inflammation and oncogenesis. Antiinflammatory activities of green tea extract (GTE) protect against dietary high-fat (HF)-induced NASH. I hypothesized that antiinflammatory and anti-oncogenic activities of GTE during NASH would also prevent diethylnitrosamine (DEN)-induced development towards HCC. Male C57BL/6J mice (4 wk old) were fed a HF diet devoid of, or supplemented with, GTE at 2% (w/w) and received once weekly intraperitoneal injections of saline vehicle or DEN (60 mg/kg; 5 and 7 wk old) until 25 wk old. Gross pathological observation indicated no tumors, as expected. GTE protected against obesity-associated parameters, histological and biochemical evidence of NASH, and hepatic TNFa and MCP-1 expression in both saline- and DEN-injected mice (P<0.05). GTE attenuated serum alanine aminotransferase (ALT) activity, hepatic malondialdehyde (MDA), hepatic iNOS and survivin mRNA expression, signal transducer and activator of transcription 3 (STAT3), and hepatocyte proliferating cell nuclear antigen (PCNA) otherwise exacerbated by DEN. Hepatic GSTP protein expression increased in mice fed GTE. Serum ALT was correlated (r = 0.71-0.83; P<0.0001) with MDA, iNOS, STAT3, survivin and PCNA. iNOS correlated with PCNA, STAT3 (r = 0.64-0.65; P<0.05) and survivin (r = 0.66; P<0.0001), suggesting that iNOS-induced inflammation regulates liver injury and oncogenic cell proliferation. GTE lowers NASH- and DEN-mediated HCC risk by increasing GSTP and attenuating iNOS-mediated liver injury and survivin-mediated cell proliferation.
Richard Bruno, Ph.D., RD (Advisor)
Ouliana Ziouzenkova, Ph.D. (Committee Member)
Amanda Bird, Ph.D. (Committee Member)
97 p.
Nutrition
diethylnitrosamine; green tea; hepatocellular carcinoma; nonalcoholic steatohepatitis; obesity

Recommended Citations

Citations

  • Kim, J. B. (2017). Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724

    APA Style (7th edition)

  • Kim, Joshua. Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724.

    MLA Style (8th edition)

  • Kim, Joshua. "Green tea extract protects against diethylnitrosamine-mediated liver injury and cell proliferation by attenuating STAT3 and iNOS expression in high fat-induced obese mice with nonalcoholic steatohepatitis." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1502898913421724

    Chicago Manual of Style (17th edition)

osu1502898913421724
495
© 2017, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.