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Histomorphometric Analysis of Elderly Ribs at Various Locations

Crowe, Nicole M, Crowe
http://rave.ohiolink.edu/etdc/view?acc_num=osu1503304250839703

Abstract Details

2017, Master of Science, Ohio State University, Anatomy.
The purpose of this study is multifaceted: (1) to investigate how much histological structures vary between seriated sections (2) to observe and analyze linear microcracks along the curve length of elderly ribs by examining the posterior, middle, and anterior locations in an attempt to understand how bone microdamage accumulates under normal loading conditions. For this study, a total of 45 histological sections (~100 µm in thickness), stained with basic fuchsin hydrochloride were obtained from a single left or right 6th rib from five male post-mortem human subjects. Three seriated sections were taken from each location: posterior (25%), middle (50%), and anterior (75%), resulting in a total of nine sections from each individual. Data were collected via rib sections imaged at 10x magnification in bright field and observing sections on a microscope with both bright field and fluorescent capabilities at a magnification of 20x. The following histological variables were quantified and analyzed: Total Area (Tt.Ar), Endosteal Area (Es.Ar), Cortical Area (Ct.Ar), Percent Cortical Area (%Ct.Ar), Porosity Area (Po.Ar), Percent Porosity (% Po.Ar), Bone Area (B.Ar), Crack Number (Cr.N), Crack Length (Cr.Le), Crack Density (Cr.Dn), and Crack Surface Density (Cr.S.Dn). Repeated measures ANOVA results revealed no significant differences exist between seriated sections at each rib location for any of the variables. These findings suggest it may be appropriate to only analyze one section in future studies. Secondly, ANOVA results also indicate that significant locational differences exist for Cr.Le and Cr.Dn. This preliminary study suggests that crack lengths are longest in the posterior sections while crack density values are largest in the middle sections. Future investigations should incorporate the spatial distribution of microdamage when assessing how linear microcracks contribute to fracture risk in the rib.
Amanda Agnew (Advisor)
John Bolte (Committee Member)
Mark Hubbe (Committee Member)
72 p.
Anatomy and Physiology; Biology; Biomechanics
Bone Histology; Ribs; Biomechanics

Recommended Citations

Citations

  • Crowe, Crowe, N. M. (2017). Histomorphometric Analysis of Elderly Ribs at Various Locations [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1503304250839703

    APA Style (7th edition)

  • Crowe, Crowe, Nicole. Histomorphometric Analysis of Elderly Ribs at Various Locations. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1503304250839703.

    MLA Style (8th edition)

  • Crowe, Crowe, Nicole. "Histomorphometric Analysis of Elderly Ribs at Various Locations." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1503304250839703

    Chicago Manual of Style (17th edition)

osu1503304250839703
116
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This open access ETD is published by The Ohio State University and OhioLINK.