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Multi-Parameter Fluorescent Analysis and Quantitative Magnetophoresis Study as Two Different Technologies to Detect and Characterize Cells and Its Various Applications as Biomarkers

Park, Kyoung-Joo Jenny

Abstract Details

2017, Doctor of Philosophy, Ohio State University, Chemical Engineering.
Cancer is a disease affecting the global population. Especially, cancer becomes truly deadly and unstoppable when it metastasizes. Various cancer biomarkers are being developed and applied into clinical practices, but there are still many different obstacles and challenges in the field that need to be overcome. In this study, two different technologies are used to detect, characterize, and separate cancer cells and various types of cells and organisms. First one is circulating tumor cell (CTC) technology, which is composed of red blood cell lysis, negative depletion method, and integrated staining of immunocytochemical staining and RNA in situ hybridization. The second technology is single cell magnetophoresis (SCM), which measures and analyzes the magnetophoretic mobility induced by the external magnetic field created by the specially-designed magnet. First technology enables us to assess many different markers at once at protein and nucleic acid levels on a single cell basis, whereas the second technology enables us to utilize the intrinsic magnetic susceptibility of the individual cell and use the characteristics in various applications. Applying the CTC technology developed and optimized in our lab, HPV 16 expressions are characterized in HPV-positive squamous cell carcinoma of the head and neck, and the effects of different PARP-inhibitor dosing levels are investigated in triple negative breast cancer (TNBC) patients. Understanding CTCs, the major key players in metastasis, and using those discoveries as a form of biomarker is important because it can help us understand more about metastasis and cancer and also provide guidance in decision-making and evaluation. Applying the SCM technology, intrinsic magnetic susceptibility of algae and cancer cells are quantitatively analyzed to understand iron and glucose metabolism in cancerous cells. Furthermore, based on the differences in iron regulation in stem-like cells and non-stem tumor cells, label-less separation is performed, showing huge potential of iron contents and iron regulation to serve as the biomarker specific for stem-like cancer cells.
Jeffrey Chalmers (Advisor)
220 p.

Recommended Citations

Citations

  • Park, K.-J. J. (2017). Multi-Parameter Fluorescent Analysis and Quantitative Magnetophoresis Study as Two Different Technologies to Detect and Characterize Cells and Its Various Applications as Biomarkers [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1512018964871473

    APA Style (7th edition)

  • Park, Kyoung-Joo Jenny. Multi-Parameter Fluorescent Analysis and Quantitative Magnetophoresis Study as Two Different Technologies to Detect and Characterize Cells and Its Various Applications as Biomarkers. 2017. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1512018964871473.

    MLA Style (8th edition)

  • Park, Kyoung-Joo Jenny. "Multi-Parameter Fluorescent Analysis and Quantitative Magnetophoresis Study as Two Different Technologies to Detect and Characterize Cells and Its Various Applications as Biomarkers." Doctoral dissertation, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1512018964871473

    Chicago Manual of Style (17th edition)