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Yan Yuan Dissertation Final.pdf (2.59 MB)
ETD Abstract Container
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Potential roles of lutein in mammary gland development and breast cancer cell growth
Author Info
Yuan, Yan
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1513374861687537
Abstract Details
Year and Degree
2018, Doctor of Philosophy, Ohio State University, Human Ecology: Human Nutrition.
Abstract
Breast cancer is the most common form of malignancy and the second leading cause of cancer mortality in women. The most recent epidemiological studies have shown an inverse relationship between blood lutein concentration and breast cancer risk. However, it is currently unclear whether it is lutein itself, or a metabolite of lutein that plays a role in breast tissue protection. To better understand the impact of dietary lutein on breast development, we performed an in vivo feeding study by using a modified AIN-93G diet, in which lutein was added to reach the concentration 0.01%. C57/BL6 virgin female mice at 3-week of age were fed with diets with or without lutein supplementation. Plasma, mammary gland, and liver were collected after 5 weeks, 10 weeks, and 15 weeks of feeding. Plasma lutein levels were determined by high-performance liquid chromatography (HPLC). Whole mount staining, light microscopy, and immunohistochemistry (IHC) analyses were conducted to evaluate the development of mammary glands. Our results showed that plasma lutein concentration was close to human plasma lutein level, and lutein accumulated in mammary glands in the lutein supplemented group. In the control group no lutein was detected in plasma, nor mammary gland. Our data also showed that there was no difference with regards to branching of the glands, adipocyte area, or epithelial cell proliferation between the two dietary groups. In addition, to investigate the direct effects of lutein on breast cancer cellular growth, we performed an in vitro study by using multiple human breast cancer cell lines (T47D, SKBR3, ZR-751, MCF7, MDA-MB-231). The cells were treated with lutein or ß-carotene at the concentrations ranging from 0.1 µM to 10 µM, followed by assessing one measurement of cell proliferation and the uptake of carotenoid by the cells. Our in vitro studies showed that there was no significant effect of lutein on breast cancer cell growth with each concentrations we tested. ß-Carotene, used as a positive control, led to around 30% decrease in cell growth at 1.0 µM and 10 µM. All five human breast cancer cell lines accumulated lutein to a greater extent than ß-carotene. Taken together, our data indicate that there is no effect of lutein on normal mouse breast development or human cancer cell growth. The association between plasma lutein and breast cancer risk described in previous studies might result from lutein being a biomarker of vegetable intake, or a marker of other phytochemical intake, and not a direct effect of lutein itself.
Committee
Earl Harrison (Advisor)
Pages
127 p.
Subject Headings
Nutrition
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Citations
Yuan, Y. (2018).
Potential roles of lutein in mammary gland development and breast cancer cell growth
[Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1513374861687537
APA Style (7th edition)
Yuan, Yan.
Potential roles of lutein in mammary gland development and breast cancer cell growth.
2018. Ohio State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1513374861687537.
MLA Style (8th edition)
Yuan, Yan. "Potential roles of lutein in mammary gland development and breast cancer cell growth." Doctoral dissertation, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1513374861687537
Chicago Manual of Style (17th edition)
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Document number:
osu1513374861687537
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663
Copyright Info
© 2018, some rights reserved.
Potential roles of lutein in mammary gland development and breast cancer cell growth by Yan Yuan is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.