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Utilization of Synbiotics, Acidifiers, and a Polyanhydride Nanoparticle Vaccine in Enhancing the Anti-Salmonella Immune Response in Laying Hens Post-Salmonella Challenge

Markazi, Ashley
http://rave.ohiolink.edu/etdc/view?acc_num=osu1521720140828545

Abstract Details

2018, Doctor of Philosophy, Ohio State University, Animal Sciences.
Salmonellosis, a zoonotic disease caused by the bacterium Salmonella, is most commonly attributed to the consumption of poultry eggs and meat. The current project examined the effects of drinking water synbiotics, in-feed acidifiers, and a polyanhydride nanoparticle Salmonella vaccine in enhancing the anti-Salmonella immune response and decreasing Salmonella infection in laying hens. The synbiotic experiment was conducted to study the effects of drinking water supplementation of synbiotic product in laying hens with and without a Salmonella challenge. A total of 384 one-day-old layer chicks were randomly distributed to the drinking water synbiotic supplementation or control groups. At 14 wk of age, the birds were vaccinated with a Salmonella vaccine, resulting in a 2 (control and synbiotic) X 2 (non-vaccinated and vaccinated) factorial arrangement of treatments. At 24 wk of age, half of the birds in the vaccinated groups and all the birds that were not vaccinated were challenged with Salmonella Enterica serotype Enteritidis, resulting in a 3 (vaccinated, challenged, vaccinated+challenged) X 2 (control and synbiotic) factorial arrangement. At 8 d post-Salmonella challenge, synbiotic supplementation decreased (P = 0.04) cecal S. Enteritidis in the challenge group compared to the un-supplemented challenge group. At 17 d post-Salmonella challenge, synbiotic supplementation increased bile anti-Salmonella IgA in the challenge group compared to the birds in the challenge group without synbiotic supplementation. The acidifier experiment was conducted to study the effects of acidifier supplementation in laying hens with and without a Salmonella challenge. A total of 384 one-day-old layer chicks were randomly distributed to the acidifier supplementation or control groups. At 14 wk of age, the birds were vaccinated with a Salmonella vaccine, resulting in a 2 (control and acidifier) X 2 (unvaccinated and vaccinated) factorial arrangement. At 24 wk of age, half of the birds in the vaccinated groups and all the birds that were not vaccinated were challenged with Salmonella Enterica serotype Enteritidis, resulting in a 3 (vaccinated, challenged, vaccinated+challenged) X 2 (control and acidifier) factorial arrangement. At 8 d post-Salmonella challenge, birds supplemented with acidifier in the challenge group had decreased (P = 0.04) cecal S. Enteritidis percentage compared to the challenge group without acidifier supplementation. At 22, 24, and 30 d post-Salmonella challenge, acidifier supplementation had higher (P < 0.01) plasma anti-Salmonella IgA titers in the challenge group compared to the challenge group without acidifier supplementation. The polyanhydride nanoparticle experiment analyzed the immunological effects of an oral Salmonella nanoparticle vaccine (OMPs-F-PNPs) loaded with Salmonella outer membrane proteins (OMPs) and flagellin proteins. At 6 wk of age, chickens were either orally vaccinated with PBS (Mock), soluble OMPs (0.05 mg) + flagellin (0.05 mg) in PBS (Antigen), or OMPs (0.05 mg) + flagellin (0.05 mg) loaded OMPs-F-PNPs suspension in 1 mL of sterile PBS (OMPs-F-PNPs). The same dose and route of delivery were repeated at 9 and 12 wk of age. At 15 wk of age, chickens were challenged with 1 x 109 CFU of live S. Enteritidis. Birds that were vaccinated with OMPs-F-PNPs had significantly (P < 0.01) higher serum and bile OMPs-specific IgG titers compared to that from the mock + challenge and the mock treatment groups. The OMPs-F-PNPs vaccinated birds had significantly fewer positive samples for cecal S. Enteritidis compared to that of the mock + challenge group. In conclusion, drinking water synbiotic supplementation, in-feed acidifier supplementation, and the polyanhydride Salmonella nanoparticle vaccine each resulted in significant increases in the anti-Salmonella immune response as measured by Salmonella-specific antibody titers and decreased Salmonella colonization in layer chickens.
Ramesh Selvaraj (Advisor)
Michael Lilburn (Advisor)
Lisa Bielke (Committee Member)
Aradhya Gourapura (Committee Member)
176 p.
Animal Sciences
Salmonella; vaccine; probiotic; acidifier

Recommended Citations

Citations

  • Markazi, A. (2018). Utilization of Synbiotics, Acidifiers, and a Polyanhydride Nanoparticle Vaccine in Enhancing the Anti-Salmonella Immune Response in Laying Hens Post-Salmonella Challenge [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1521720140828545

    APA Style (7th edition)

  • Markazi, Ashley. Utilization of Synbiotics, Acidifiers, and a Polyanhydride Nanoparticle Vaccine in Enhancing the Anti-Salmonella Immune Response in Laying Hens Post-Salmonella Challenge. 2018. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1521720140828545.

    MLA Style (8th edition)

  • Markazi, Ashley. "Utilization of Synbiotics, Acidifiers, and a Polyanhydride Nanoparticle Vaccine in Enhancing the Anti-Salmonella Immune Response in Laying Hens Post-Salmonella Challenge." Doctoral dissertation, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1521720140828545

    Chicago Manual of Style (17th edition)

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