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Understanding the stability, biological impact, and exposure markers of black raspberries and strawberries using an untargeted metabolomics approach

Teegarden, Matthew D
http://rave.ohiolink.edu/etdc/view?acc_num=osu1522335050171997

Abstract Details

2018, Doctor of Philosophy, Ohio State University, Food Science and Technology.
Pre-clinical and clinical evidence suggest that dietary berries may help prevent against the development of some chronic diseases such as cardiovascular disease and oral cancer. Berries contain hundreds to thousands of phytochemicals that are thought to act synergistically to produce a wide range of biological effects. While most of the research with berries have been performed with fresh or minimally processed products, thermally processed and stored berry products also represent important dietary sources. The overall goal of this work is to build upon our understanding of the potential role of berry phytochemicals in the prevention of chronic diseases by investigating how they exist in foods and are metabolized by the body. We hypothesize that an untargeted metabolomics approach will provide novel insights to this end. The objective of these studies are to: profile the thermal processing-induced changes in the phytochemical profile of a black raspberry nectar beverage, investigate the impact of storage on the chemistry and bioactivity of this nectar against premalignant oral cancer cells, and profile the urinary metabolome of individuals consuming strawberry-based confections. Thermal processing of black raspberries was shown to lower relative levels of some chemical features, while elevating proportionally more chemical features. Some of the elevated features were characterized as potential phenolic degradation products. The stability of several classes of black raspberry (poly)phenolics was also demonstrated. Storage of black raspberry nectar at 4 °C – 35 °C induced large amounts of chemical variation in the product, without markedly affecting its overall bioactivity. A model system that mimicked the chemistry of the stored nectar products indicted that black raspberry phytochemical degradation products may play a role in maintaining the bioactivity of the product. Untargeted metabolomics revealed a chemical signature of strawberry exposure in a free-living population following a low-anthocyanin background diet. These studies support the use of untargeted metabolomics in berry research and provide novel insight that will be useful for the future development and evaluation of berry-based functional foods.
Devin Peterson, Ph.D. (Committee Co-Chair)
Jessica Cooperstone, Ph.D. (Committee Co-Chair)
Steven Clinton, M.D., Ph.D. (Committee Member)
David Francis, Ph.D. (Committee Member)
185 p.
Food Science
metabolomics; black raspberries; strawberries; food processing; storage; exposure markers

Recommended Citations

Citations

  • Teegarden, M. D. (2018). Understanding the stability, biological impact, and exposure markers of black raspberries and strawberries using an untargeted metabolomics approach [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1522335050171997

    APA Style (7th edition)

  • Teegarden, Matthew. Understanding the stability, biological impact, and exposure markers of black raspberries and strawberries using an untargeted metabolomics approach. 2018. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1522335050171997.

    MLA Style (8th edition)

  • Teegarden, Matthew. "Understanding the stability, biological impact, and exposure markers of black raspberries and strawberries using an untargeted metabolomics approach." Doctoral dissertation, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1522335050171997

    Chicago Manual of Style (17th edition)

osu1522335050171997
538
© 2018, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.