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Kilborne_Masters Thesis 2018.pdf (753.71 KB)

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The Effects of Hyaluronan Alone or in Combination with Chondroitin Sulfate and N-Acetyl-D-Glucosamine on Lipopolysaccharide-Challenged Equine Fibroblast-like Synovial Cells

Kilborne, Allison
http://rave.ohiolink.edu/etdc/view?acc_num=osu1522336928331511

Abstract Details

2018, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
Objective: To investigate effects of hyaluronic acid (HA) or HA combined with chondroitin sulfate (CS) and N-acetyl-d-glucosamine (NAG) by use of a lipopolysaccharide (LPS) in vitro method. Sample: Monolayer cultures of synovial cells from 4 adult horses. Procedure: Synovial cell cultures were untreated or treated with HA alone or HA- CS-NAG for 24 hours, subsequently unchallenged or challenge-exposed with 2 LPS concentrations (20 and 50 ng/mL) for 2 hours, and retreated with HA or HA-CS-NAG for another 24 hours. Cellular morphology of cultures was evaluated at 0, 24 (before LPS), 26 (after LPS), and 50 (24 hours after end of LPS) hours. At 50 hours, cell number and viability and prostaglandin (PG) E2, interleukin (IL)-6, matrix metalloproteinase (MMP)3, and cyclooxygenase (COX)-2 production were measured. Results: LPS challenge exposure induced a significant loss of characteristic synovial cell morphology, decrease in cell viability, and increases in concentrations of PGE2, IL-6, MMP-3, and COX-2. Cells treated with HA or HA-CS-NAG had significantly better viability and morphology scores and lower concentrations of PGE2, MMP-3, IL-6, and COX-2 than untreated LPS challenge-exposed cells. Cells treated with HA had significantly better morphology scores at 50 hours than cells treated with HA-CS-NAG. Cells treated with HA-CS-NAG had significantly superior suppression of LPS-induced production of PGE2, IL-6, and MMP-3 than cells treated with HA alone. Conclusion and Clinical Relevance: HA and HA-CS-NAG protected synovial cells from the effects of LPS. Treatment with HA-CS-NAG had the greatest anti-inflammatory effect. These results supported the protective potential of HA and HA-CS-NAG treatments.
Alicia Bertone (Advisor)
Matthew Brokken (Committee Member)
Prosper Boyaka (Committee Member)
25 p.
Veterinary Services
hyaluronan, polyglycan, osteoarthritis, equine

Recommended Citations

Citations

  • Kilborne, A. (2018). The Effects of Hyaluronan Alone or in Combination with Chondroitin Sulfate and N-Acetyl-D-Glucosamine on Lipopolysaccharide-Challenged Equine Fibroblast-like Synovial Cells [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1522336928331511

    APA Style (7th edition)

  • Kilborne, Allison. The Effects of Hyaluronan Alone or in Combination with Chondroitin Sulfate and N-Acetyl-D-Glucosamine on Lipopolysaccharide-Challenged Equine Fibroblast-like Synovial Cells . 2018. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1522336928331511.

    MLA Style (8th edition)

  • Kilborne, Allison. "The Effects of Hyaluronan Alone or in Combination with Chondroitin Sulfate and N-Acetyl-D-Glucosamine on Lipopolysaccharide-Challenged Equine Fibroblast-like Synovial Cells ." Master's thesis, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1522336928331511

    Chicago Manual of Style (17th edition)

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