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Persistence of ESBL E. coli in Poultry Production Free of Antibiotic Application

Wang, Zihua
http://rave.ohiolink.edu/etdc/view?acc_num=osu1524047763189613

Abstract Details

2018, Master of Science, Ohio State University, Food Science and Technology.
The rapid rise of antibiotic resistant pathogens has been a major public health threat. However, commensal bacteria have played key roles in antibiotic resistance (AR) development, dissemination and persistence. It is now further recognized that AR is a complicated issue and multiple risk factors contribute to the AR crisis seen today. Poultry is the largest sector of the food animal production industry, with extended history of antibiotic applications. This study examined the prevalence and abundance of AR in poultry fecal microbiota without antibiotic exposure. Multidrug resistant bacteria were abundant in poultry feces examined in the study. Multiple ESBL isolates were identified to be E. coli. Plasmids were extracted from the isolates and transferred to the antibiotic susceptive DH5a strain and led to acquired resistance. Antibiotic resistant donor strains and antibiotic resistant transformants retained the resistant phenotype after consecutive transfer in antibiotic-free medium. The data illustrated that ART bacteria were abundant in poultry gut and fecal microbiota even without direct exposure to antibiotics, and the ESBL-encoding plasmids are transferable and can be functional in transformants, and the resistant strains were persistent in the antibiotic-free poultry production as well as culture environment. Therefore, innovative strategies besides banning the use of antibiotics are needed to address the major public health challenge due to AR.
Hua Wang (Advisor)
Melvin Pascall (Committee Member)
Lynn Knipe (Committee Member)
66 p.
Food Science; Microbiology

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Citations

  • Wang, Z. (2018). Persistence of ESBL E. coli in Poultry Production Free of Antibiotic Application [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524047763189613

    APA Style (7th edition)

  • Wang, Zihua. Persistence of ESBL E. coli in Poultry Production Free of Antibiotic Application. 2018. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1524047763189613.

    MLA Style (8th edition)

  • Wang, Zihua. "Persistence of ESBL E. coli in Poultry Production Free of Antibiotic Application." Master's thesis, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524047763189613

    Chicago Manual of Style (17th edition)

osu1524047763189613
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This open access ETD is published by The Ohio State University and OhioLINK.