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Evaluation of Veterinary Allergen Extract Content and Resultant Canine Intradermal Threshold Concentrations

Abrams, Stephanie B
http://orcid.org/0000-0003-2070-6484
http://rave.ohiolink.edu/etdc/view?acc_num=osu1524066093298819

Abstract Details

2018, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
One of the challenges in veterinary aeroallergy is the variability of the aqueous allergen extracts used for intradermal testing (IDT). Optimal extract threshold concentrations (TCs) for IDT are defined as the highest concentration of allergen that results in positive reactivity in =10% of a normal population to minimize false positive and negative reactions in dogs with atopic dermatitis. Limited information is available on IDT TCs and protein composition for common allergens from different allergen extract suppliers. The objectives of this study were to survey the protein heterogeneity of tree, grass, weed, and mite allergen extract lots between and within manufacturers, and to determine IDT allergen extract TCs for healthy dogs using allergens from two veterinary allergen extract manufacturers. We hypothesized that protein composition and IDT TCs would vary according to allergen extract and manufacturer. Eleven allergens were obtained from ALK-Abello and Stallergenes Greer. Protein concentration and heterogeneity were evaluated using a Bradford-style assay and SDS-PAGE. IDT was performed in 25 privately owned, clinically healthy dogs and 10 purpose-bred beagles with the 11 allergens from each company at six dilutions. Immediate reactions were subjectively scored (0 to 4+), and objectively measured (mm) and their percent concordance was evaluated. TCs were determined by a positive reaction (=2+) at 15 minutes in combination with generalized estimating equations. Allergen extract protein quantity and composition varied within and between manufacturers despite sharing the same PNU/mL values. TCs of one weed, five trees, two grasses and a house dust mite were determined for ALK extracts, whereas TCs for three weeds, three trees and two grasses were identified for Greer extracts. Receiver operating characteristic curve analyses determined a percent concordance of the objective and subjective values of 77.3% for ALK and 75% for Greer allergens. Veterinary allergen extracts labeled with the same genus and species as well as with the same PNU/mL may not be interchangeable between manufacturers and may require different testing concentrations for use in an IDT.
Gwendolen Lorch (Advisor)
Lynette Cole (Committee Member)
Sandra Diaz (Committee Member)
164 p.
Therapy
canine atopic dermatitis; intradermal allergy testing, allergen threshold concentrations

Recommended Citations

Citations

  • Abrams, S. B. (2018). Evaluation of Veterinary Allergen Extract Content and Resultant Canine Intradermal Threshold Concentrations [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524066093298819

    APA Style (7th edition)

  • Abrams, Stephanie. Evaluation of Veterinary Allergen Extract Content and Resultant Canine Intradermal Threshold Concentrations. 2018. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1524066093298819.

    MLA Style (8th edition)

  • Abrams, Stephanie. "Evaluation of Veterinary Allergen Extract Content and Resultant Canine Intradermal Threshold Concentrations." Master's thesis, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524066093298819

    Chicago Manual of Style (17th edition)

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