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Microfluidic Technologies: Micromagnetic Doublet Dynamics and Nucleic Acid Testing

Pease, Christopher Adam

Abstract Details

2018, Doctor of Philosophy, Ohio State University, Physics.
Microfluidic technologies have been developed, over the past decade, to manipulate and detect biological materials at increasingly smaller length scales. This thesis presents one technique for micron-scale manipulation using micromagnetic particles and another technique for biological material detection through nucleic acid testing. In a magnetic field, micromangetic particles form chains and clusters. The simplest such chain is the fluid-borne doublet, where two magnetic beads are in close proximity, but remain unattached, allowing each bead to freely rotate. In a rotating magnetic field, there are magnetic torques that separately rotate the doublet and its constituent beads. The individual rotating beads apply a hydrodynamic torque on the doublet arising from the bead-doublet coupling. The first part of this thesis investigates, through experiments and simulations, the dynamics of such field-driven doublets. New dynamics were found and analytical equations derived for the average doublet rotation rate for the case where the dominant torque stems from the hydrodynamic coupling. A criteria was also determined based on the magnetic and hydrodynamic torques that distinguishes whether the doublets have a higher maximum rotation rate when the beads are attached or detached to each other. Nucleic acid testing is often used for medical diagnostics. Quadruplex Priming Amplification (QPA) is a nucleic acid amplification technique for real-time quantitative assays that is both isothermal and uses few chemical reagents. Integrating QPA with a microfluidics device will further optimize the assay by automating and combining multiple reaction steps and by reducing the quantity and cost of reagents. The second part of this thesis investigates for the first time the QPA reaction in a microfluidic chip where the reaction was able to be performed comparably to a non-microfluidics-based commercially available device.
Ratnasingham Sooryakumar (Advisor)
Ciriyam Jayaprakash (Committee Member)
Comert Kural (Committee Member)
Stanley Durkin (Committee Member)
128 p.

Recommended Citations

Citations

  • Pease, C. A. (2018). Microfluidic Technologies: Micromagnetic Doublet Dynamics and Nucleic Acid Testing [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu152570770491179

    APA Style (7th edition)

  • Pease, Christopher. Microfluidic Technologies: Micromagnetic Doublet Dynamics and Nucleic Acid Testing. 2018. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu152570770491179.

    MLA Style (8th edition)

  • Pease, Christopher. "Microfluidic Technologies: Micromagnetic Doublet Dynamics and Nucleic Acid Testing." Doctoral dissertation, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu152570770491179

    Chicago Manual of Style (17th edition)