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Clathrin-Mediated Endocytosis as a Marker of Cell Membrane Tension in Cultured Cells and Developing Organisms

Ferguson, Joshua Paul
http://orcid.org/0000-0002-7990-6387
http://rave.ohiolink.edu/etdc/view?acc_num=osu1534434899256265

Abstract Details

2018, Doctor of Philosophy, Ohio State University, Physics.
Individual cells decipher and react to both their chemical and mechanical environment. Clathrin-mediated endocytosis (CME) is a major process by which cells internalize macromolecules. The triskelion-shaped clathrin protein assembles on the membrane as a spherical lattice enveloping the membrane until scission begets internalization. The membrane curvature generated by the invaginations during endocytosis associate CME with the mechanical environment of the cell. Fluorescence microscopy is used to study the dynamics of CME, and in particular to discern the time it takes for CME events to complete (i.e. their lifetime). It is our hypothesis that the lifetime of CME events relates inversely to the cell membrane tension. We will support this hypothesis with live-cell imaging on glass substrates and in living organisms. We suggest a new methodology for studying CME dynamics that enables higher spatial and temporal resolution than lifetime analysis. We will also characterize the tension response of CME by using various cell manipulation techniques. In addition, we will demonstrate the ability of CME dynamics to predict cell movement and relate gradients in clathrin coat growth rates to previously established tension gradients in cultured cells and living organisms. Finally, we will demonstrate that this process is in a state of continual curvature generation.
Comert Kural (Advisor)
Ralph Bundschuh (Committee Member)
Michael Poirier (Committee Member)
Sooryakumar Ratnasingham (Committee Member)
136 p.
Biophysics; Physics
clathrin mediated endocytosis; membrane tension; fluorescence microscopy; biomechanics; cell biology; biophysics

Recommended Citations

Citations

  • Ferguson, J. P. (2018). Clathrin-Mediated Endocytosis as a Marker of Cell Membrane Tension in Cultured Cells and Developing Organisms [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1534434899256265

    APA Style (7th edition)

  • Ferguson, Joshua. Clathrin-Mediated Endocytosis as a Marker of Cell Membrane Tension in Cultured Cells and Developing Organisms. 2018. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1534434899256265.

    MLA Style (8th edition)

  • Ferguson, Joshua. "Clathrin-Mediated Endocytosis as a Marker of Cell Membrane Tension in Cultured Cells and Developing Organisms." Doctoral dissertation, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1534434899256265

    Chicago Manual of Style (17th edition)

osu1534434899256265
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This open access ETD is published by The Ohio State University and OhioLINK.