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Neeraja_Sundar_Rajan- Thesis.pdf (2.33 MB)

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Bio-separation of Methemoglobin and Oxyhemoglobin using Magnetic Chromatography

Sundar Rajan, Neeraja, Rajan
http://rave.ohiolink.edu/etdc/view?acc_num=osu1543271660561819

Abstract Details

2018, Master of Science, Ohio State University, Chemical Engineering.
Bio-separations using intrinsic magnetic properties of blood proteins is an exciting field of study. A hemoglobin molecule can exist in different oxidative states, the most common being oxyhemoglobin(oxy-Hb) and methemoglobin(met-Hb). Owing to the number of unpaired electrons, while oxyhemoglobin exhibits diamagnetism, methemoglobin exhibit paramagnetic behavior. This contrasting magnetic property can be used to separate the two species using magnetic chromatography. Magnetic chromatography techniques utilize magnetic microparticles (iron microparticles or nanoparticles) to maintain a high magnetic gradient, which in turn helps to separate compounds based on their magnetic susceptibility. To test this, in place of hemoglobin, highly magnetic super paramagnetic iron oxide nanoparticles (SPIONS) of diameter 5 nanometers dissolved in chloroform was placed in a column with iron microparticles. This column was placed in the magnetic field to study their diffusion. It was observed that the concentration of SPIONS in the solution was reduced within a span of thirty minutes. The flow through collected from the column was much lighter in color. The challenge is that due to its highly reactive nature, hemoglobin reacts with these magnetic microparticles and forms a complex precipitate, making separation of the hemoglobin species impractical. Initially, a hollow glass column was designed, and it consisted of an equimolar mixture of hemoglobin species and magnetic iron nanoparticles inside its annulus. This column was placed in a Quadrupole Magnetic System (QMS) whose magnetic field intensity is up to 2.5 Tesla. Due to the direct physical contact of the iron nanoparticles with the hemoglobin complex precipitate was formed. To overcome this, the electrospinning technique was employed to coat the nanoparticles with polyacrylonitrile (PAN). The nanofiber mat designed this way was placed in a magnetic field and an equimolar mixture of hemoglobin species was passed through the separator. This proposal focuses on achieving higher yield of separation by designing a suitable separator and optimizing the nanofiber mat properties. Details of the background, experimental techniques, simulations of the processes, results and new insights are included in this thesis.
Jeffrey Chalmers (Advisor)
Andre Palmer (Committee Member)
59 p.
Chemical Engineering
Magnetic Chromatography; Quadrupole Magnetic System; SPOINS; Hemoglobin purification; Quadrupole field; Magnetic colloid;

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Citations

  • Sundar Rajan, Rajan, N. (2018). Bio-separation of Methemoglobin and Oxyhemoglobin using Magnetic Chromatography [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1543271660561819

    APA Style (7th edition)

  • Sundar Rajan, Rajan, Neeraja. Bio-separation of Methemoglobin and Oxyhemoglobin using Magnetic Chromatography. 2018. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1543271660561819.

    MLA Style (8th edition)

  • Sundar Rajan, Rajan, Neeraja. "Bio-separation of Methemoglobin and Oxyhemoglobin using Magnetic Chromatography." Master's thesis, Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1543271660561819

    Chicago Manual of Style (17th edition)

osu1543271660561819
578
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