Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

Product Development, Sensory Evaluation and Characterization of Bioactive Isothiocyanates from Broccoli Sprout Powder delivered in Tomato Juice

Rodriguez, Carla Jeanette

Abstract Details

2019, Master of Science, Ohio State University, Food Science and Technology.
Previous studies have found carotenoids in tomato juice (TJ), and isothiocyanates in broccoli to be associated with positive health outcomes such as bladder cancer prevention. These foods should be investigated further but from previous attempts, there were some reported challenges including lack of flavor and sensory acceptability. Using a sous vide method, with a specific time and temperature, we hypothesized that the development of a product with maximal amount of isothiocyanates and high organoleptic quality was achievable. A multi-characterization approach was employed in analyzing microstructural properties, bioactive present and sensorial acceptability of pretreated broccoli sprout powder (BSP) in TJ. The product was designed to contain 1 gram of broccoli sprout powder which is equivalent to a serving of mature broccoli. The first group of fresh broccoli sprouts were washed, dried and vacuum sealed, and sous vide (Model SVS-10LS, CO) at 60˚C for 10 minutes. The second group was the raw control broccoli sprouts which were washed, rinsed and patted dry. The third group was the enzyme inactivated steamed broccoli sprouts, this group was washed and placed on a sieve to be steamed for 5 minutes. All the samples were placed on their individual trays, flash frozen with liquid nitrogen and freeze dried for 48 hours. After lyophilization, the samples were pulverized into a powder, placed in labeled plastic bags and stored in a low humidity environment at 4˚C until analyses. Rheological temperature-dependence studies between 25-65 ˚C were conducted to assess impact of 1 gram of BSP in 6 oz of TJ (GFS, Columbus, OH) on the rheological properties including complex viscosity, elastic modulus and loss modulus. The quantification of these rheological properties allows us to have an understanding of how the flow of the TJ will be perceived by humans. HPLC analysis was used to determine and compare levels of glucosinolates as well as the levels of bioactive isothiocyanates. Particle size, polydispersity, and zeta potential were determined using dynamic light scattering particle analyzer. Sensory panel tests were performed to determine consumer acceptability using hedonic scale, JAR and preference test. A Dynamic Vapor Sorption isotherm was generated to observe water interaction with the powder at different levels of relative humidity for determining optimal storage conditions. The rheological testing showed that at the proposed dose, there was a not a significant difference in the complex viscosity, elastic and loss modulus values between 6 oz of plain TJ compared to 6 oz TJ with 1 g of BSP. The overlapping of the results demonstrates there was not a detectable difference of the addition of BSP in the viscosity or flow of TJ. Dynamic tests as a function of temperature evidenced the stability and the compatibility between tomato juice and broccoli sprout powder. Levels of glucosinolates by HPLC/MS resulted in 50.79µmol/g in steamed BSP, 1.86µmol/g for the sous vide BSP and 19.75µmol/g for the raw BSP. Levels of isothiocyanates by HPLC/MS resulted in 9.2 µmol/g in steamed BSP, 39.4 µmol/g for the sous vide and 14.2 µmol/g for the raw control. Epidemiology has shown that consumption of raw broccoli seems to have a protective effect from bladder cancer, by consuming sous vide BSP the effect should be almost three times greater. This data serves as indication that level of bioactive isothiocyanates can be maximized with sous vide processing as compared to the raw and steamed BSP. Dynamic light scattering data shows the sous vide BSP particles in a size range between 600nm and 9000nm with a polydispersity value of 0.513. This polydispersity value is over the 0.5 threshold for achieving a homogeneous particle size, which could be optimized with a better controlled grinding method. Results of a sensory panel showed significantly greater acceptability than past studies with an overall hedonic scale score of 5.2 for canned tomato juice with BSP. Sensory test results from hedonic scale and JAR test were expected to have consistently higher acceptability from plastic bottled tomato juice with BSP as compared to canned. Furthermore, blinded preference test revealed 65% percent of the panelists preferred the canned TJ with sous vide BSP over plastic bottled TJ with the same sous vide BSP. Results suggest the proposed processing method is effective in achieving a palatable TJ with BSP that delivers a significant concentration of bioactive isothiocyanates. Physicochemical evaluation of TJ with BSP confirmed stability and compatibility between TJ and BSP.
Yael Vodovotz, Ph.D. (Advisor)
Christopher Simons, Ph.D. (Committee Member)
Clinton Steven, M.D., Ph.D. (Committee Member)
76 p.

Recommended Citations

Citations

  • Rodriguez, C. J. (2019). Product Development, Sensory Evaluation and Characterization of Bioactive Isothiocyanates from Broccoli Sprout Powder delivered in Tomato Juice [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1544800823179656

    APA Style (7th edition)

  • Rodriguez, Carla. Product Development, Sensory Evaluation and Characterization of Bioactive Isothiocyanates from Broccoli Sprout Powder delivered in Tomato Juice. 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1544800823179656.

    MLA Style (8th edition)

  • Rodriguez, Carla. "Product Development, Sensory Evaluation and Characterization of Bioactive Isothiocyanates from Broccoli Sprout Powder delivered in Tomato Juice." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1544800823179656

    Chicago Manual of Style (17th edition)