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On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension

Huber, Scott David
http://rave.ohiolink.edu/etdc/view?acc_num=osu1545921340769193

Abstract Details

2019, Doctor of Philosophy, Ohio State University, Physics.
Clathrin-mediated endocytosis (CME) is a process in which plasma membrane area is transferred from the surface of the cell to the cell interior. Because cell volume increases and cell shape stays roughly constant in this process, the reduction in surface area must be accompanied by work done against the membrane’s elasticity. This work is directly dependent on the tension of the cell membrane. From this, we hypothesize that CME dynamics will be totally or partially inhibited upon an increase in membrane tension. We prove this hypothesis in vitro and in vivo, utilizing newly developed methods for the quantification of CME dynamics. We then test the converse of this result—that impaired CME dynamics imply elevated membrane tension—to assess CME as an indirect reporter of spatial and temporal heterogeneities in membrane tension. Additionally, we assess the role of several CME-associated proteins in counteracting tension.
Comert Kural (Advisor)
Michael Poirier (Committee Member)
Michael Lisa (Committee Member)
Ralf Bundschuh (Committee Member)
121 p.
Cellular Biology; Physics
Clathrin; Endocytosis; Tension; CALM; PICALM; Fluorescent microscopy

Recommended Citations

Citations

  • Huber, S. D. (2019). On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1545921340769193

    APA Style (7th edition)

  • Huber, Scott. On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension . 2019. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1545921340769193.

    MLA Style (8th edition)

  • Huber, Scott. "On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension ." Doctoral dissertation, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1545921340769193

    Chicago Manual of Style (17th edition)

osu1545921340769193
251
© 2019, some rights reserved.Creative Commons License On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension by Scott David Huber is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.