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Movement of the Male Germ Unit in Pollen Tubes

Hance, Elizabeth

Abstract Details

2019, Master of Mathematical Sciences, Ohio State University, Mathematics.
During reproduction a pollen tube grows to make a path for the male germ unit, composed of a vegetative nucleus and two sperm cells, to move to the egg for fertilization. One of the sperm cells has a cytoplasmic projection that connects to the vegetative nucleus. It is suspected the vegetative nucleus and sperm cells have connections to the pollen tube cytoskeletal system which allows for movement within the tube. We begin by extracting important features from videos of male germ unit movement and from kymograph data. To analyze the kymograph images, we calculate the center of light intensity of the vegetative nucleus and sperm cells. We then analyze our data, extract the size of various quantities, and attempt to fit a probability model to the positive/negative movement of the male germ unit. Finally, we build a biophysical model by modeling the connection between the vegetative and sperm cells with a linear spring. We show preliminary results of the trajectories created with this model. This thesis is partially supported by the NSF CAREER Award 1553637.
Chuan Xue (Advisor)
Iris Meier (Committee Member)
Adriana Dawes (Committee Member)
64 p.

Recommended Citations

Citations

  • Hance, E. (2019). Movement of the Male Germ Unit in Pollen Tubes [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1554804205187184

    APA Style (7th edition)

  • Hance, Elizabeth. Movement of the Male Germ Unit in Pollen Tubes. 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1554804205187184.

    MLA Style (8th edition)

  • Hance, Elizabeth. "Movement of the Male Germ Unit in Pollen Tubes." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1554804205187184

    Chicago Manual of Style (17th edition)