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Production of Heavy Oil Liamocins by Aureobasidium pullulans

Qin, Zhen
http://orcid.org/0000-0002-6961-185X
http://rave.ohiolink.edu/etdc/view?acc_num=osu1555426343947633

Abstract Details

2019, Master of Science, Ohio State University, Food Science and Technology.
Used to be known as heavy oil, liamocins are extracellular lipids product by specific Aureobasidium pullulans strains isolated from the diverse natural environment at nitrogen limited condition. Liamocins have many potential applications in food, pharmaceutical, cosmetical and chemical industries. Crude heavy oil can be produced in diverse colors, varying from pale pink, light yellow to dark brown and dark olivaceous. Typical producer A. pullulan NRRL 62031 can produce yellow liamocins with negligible byproduct melanin with titer of 29.5g/L and yield of 0.30 g/g in a medium containing 12% (w/v) glucose and C/N of 175. Xylose can also be utilized to converse liamocins by A. pullulans. With different loading volume from 30 mL to 150 mL of culture in 250 mL shake flasks, 30.5 g/L liamocins titer was attained in 30 mL culture. Novel liamocins producing strains A. pullulan NRRL 58551, 58544 and 62031 in 11-day fermentation shows that liamocins were produced when cells stopped multiplication, which indicates that liamocins are secondary metabolites. Fermentation kinetics also revealed yields at stationary phase of 0.29, 0.29 and 0.19 g/g. Fed-batch fermenation in shake flasks of initial glucose concentration started at 40 g/L and 80 g/L fed with concentrated glucose solution 2 times and 1 time attained 28.1 g/L and 27.1 g/L titers. Fermentation was scaled up in 5-L stirred tank bioreactor with 0.20 g/g yield from glucose in 216-hour fermentation. The simulation and economic analysis of liamocins industrial production indicate a cost of $3.18 /kg liamocins produced. And the industrial production could be paid back in 3.27 years.
Shang-Tian Yang (Advisor)
Ahmed Yousef (Committee Member)
Hua Wang (Committee Member)
67 p.
Food Science

Recommended Citations

Citations

  • Qin, Z. (2019). Production of Heavy Oil Liamocins by Aureobasidium pullulans [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555426343947633

    APA Style (7th edition)

  • Qin, Zhen. Production of Heavy Oil Liamocins by Aureobasidium pullulans. 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1555426343947633.

    MLA Style (8th edition)

  • Qin, Zhen. "Production of Heavy Oil Liamocins by Aureobasidium pullulans." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555426343947633

    Chicago Manual of Style (17th edition)

osu1555426343947633
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This open access ETD is published by The Ohio State University and OhioLINK.