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NW_M.S. Thesis_v6.pdf (3.42 MB)
ETD Abstract Container
Abstract Header
Resurrection of Organophosphorus-Aged Acetylcholinesterase via Mannich Bases Derived from Proline
Author Info
Ward, Nathan Andrew
ORCID® Identifier
http://orcid.org/0000-0002-9856-3607
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1565714137331736
Abstract Details
Year and Degree
2019, Master of Science, Ohio State University, Chemistry.
Abstract
Organophosphorus (OP) compounds are responsible for an estimated 220,000 deaths annually. OP compounds inhibit the enzyme acetylcholinesterase (AChE) via phosphylation of a serine residue within the active site. Upon inhibition of AChE, acetylcholine accumulates in neuromuscular junctions, resulting in a cholinergic crisis and, if not treated immediately, can lead to death. Along with management of symptoms, current medical countermeasures for OP poisoning utilize nucleophilic oximes, which can displace the phosphorus moiety from the serine residue, reactivating AChE to its native state. However, following inhibition, a spontaneous O-dealkylation of the phosphylated serine residue can occur, resulting in an anionic phosphylated serine residue; this process is known as aging. There is currently no approved therapeutic that is capable of restoring OP-aged AChE to its native state (a process referred to as resurrection). Previous studies by our group have shown that a class of compounds called quinone methide precursors (QMPs) both resurrect OP-aged AChE and reactivate OP-inhibited AChE back to its native state. A previous lead QMP from the Hadad group featured a pyrrolidine leaving group, while the current lead QMP features an (R)-2-methylpyrrolidine leaving group. Thus, the addition of a methyl group at the 2-position of the pyrrolidine ring with the (R)-configuration increased the resurrection efficacy of OP-aged AChE. This work describes the synthesis of novel QMPs that have been derived from proline. Proline was derivatized to vary the functional group at the 2-position of the pyrrolidine ring in an effort to further increase the resurrection efficacy of OP-aged AChE. More specifically, proline was derivatized to feature ester, amide, alcohol, ether, and amine functional groups. Then, the QMPs were synthesized via a Mannich reaction between 3-hydroxypyridine and the proline derivatives. The QMPs were screened against OP-aged AChE and the resurrection efficacy was measured via Ellman’s assay.
Committee
Christopher Hadad, Ph.D. (Advisor)
Jon Parquette, Ph.D. (Committee Member)
Pages
122 p.
Subject Headings
Biochemistry
;
Chemistry
;
Organic Chemistry
Keywords
organophosphorus
;
resurrection
;
proline
;
Mannich bases
;
resurrection of acetylcholinesterase
;
organophosphorus-aged
;
OP-aged AChE
Recommended Citations
Refworks
EndNote
RIS
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Citations
Ward, N. A. (2019).
Resurrection of Organophosphorus-Aged Acetylcholinesterase via Mannich Bases Derived from Proline
[Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1565714137331736
APA Style (7th edition)
Ward, Nathan.
Resurrection of Organophosphorus-Aged Acetylcholinesterase via Mannich Bases Derived from Proline.
2019. Ohio State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1565714137331736.
MLA Style (8th edition)
Ward, Nathan. "Resurrection of Organophosphorus-Aged Acetylcholinesterase via Mannich Bases Derived from Proline." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1565714137331736
Chicago Manual of Style (17th edition)
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Document number:
osu1565714137331736
Download Count:
165
Copyright Info
© 2019, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.