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Thompson MS Thesis Final Document.pdf (1.33 MB)

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Characterizing the Chondrodystrophic Canine Intervertebral Disc in Health and Disease

Thompson, Kelly
http://rave.ohiolink.edu/etdc/view?acc_num=osu157428888276191

Abstract Details

2019, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
Intervertebral disc (IVD) degeneration (IVDD) is an important cause of low back pain in people, but can be difficult to treat, either surgically or medically, with current available therapies. Thus, understanding the pathological mechanisms of IVDD and developing novel treatments are critical for improving outcome and quality of life in people living with LBP. The chondrodystrophic (CD) canine clinical model of IVDD is a promising spontaneous model through which to assess IVD-associated spinal pain and translational therapeutic strategies for LBP. The canine IVD is larger than most other small animal IVDD models and undergoes maturational changes similar to those of the human IVD. Furthermore, both dogs and people develop painful IVDD as a spontaneous process, resulting in similar characteristic pathologies and clinical signs. Future exploration of the canine as a model of IVD-associated spinal pain and biological treatments using the canine clinical model will further demonstrate its translational capabilities with the added ethical benefit of treating an existing veterinary patient population with IVDD. The goal of this study was to explore the pathological differences between herniated surgical (n=16) and healthy autopsy (n=6) CD canine nucleus pulposus (NP) samples using histology and immunohistochemistry for CD31, tryptase, and CD163 to assess blood vessel, MC, and MØ presence, respectively. Histology revealed that, compared to healthy autopsy NP, surgical NP contained smaller dispersions of NP cell clusters with large amounts of granulation tissue. While healthy NP extracellular matrix is mainly composed of aggrecan, surgical NP matrix is primarily collagen. 11/15 (73.3%) surgical samples stained positive with CD31 for blood vessels in granulation tissue, with 14/15 (93.3%) samples containing CD31-positive cells within NP tissue, versus 0/4 autopsy NP tissue samples. 12/15 (80%) of surgical samples stained positive for tryptase, with 14/15 (93.3%) containing tryptase-positive cells within NP tissue versus 0/6 autopsy NP tissue samples. 15/16 (93.8%) of surgical samples stained positive for MØ marker CD163, with 16/16 (100%) samples containing CD163-positive cells within NP tissue, versus 2/4 autopsy NP tissue samples. Thus, CD canine IVDD is characterized by blood vessel and immune cell invasion, important factors for understanding IVDD pathogenesis and developing novel restorative therapies.
Sarah Moore (Advisor)
Devina Purmessur (Advisor)
Alan Litsky (Committee Member)
65 p.
Veterinary Services
intervertebral disc; chondrodystrophic canine; low back pain; intervertebral disc degeneration; nucleus pulposus; blood vessels; mast cells; macrophages

Recommended Citations

Citations

  • Thompson, K. (2019). Characterizing the Chondrodystrophic Canine Intervertebral Disc in Health and Disease [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu157428888276191

    APA Style (7th edition)

  • Thompson, Kelly. Characterizing the Chondrodystrophic Canine Intervertebral Disc in Health and Disease. 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu157428888276191.

    MLA Style (8th edition)

  • Thompson, Kelly. "Characterizing the Chondrodystrophic Canine Intervertebral Disc in Health and Disease." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu157428888276191

    Chicago Manual of Style (17th edition)

osu157428888276191
344
© 2019, some rights reserved.Creative Commons License Characterizing the Chondrodystrophic Canine Intervertebral Disc in Health and Disease by Kelly Thompson is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.