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Ben Eggers Final Masters Thesis.pdf (2.08 MB)

ETD Abstract Container

Abstract Header

Identifying phenotypic traits critical for breeding winter malting barley adapted to Ohio and the genomic regions affecting those traits

Eggers, Ben
http://orcid.org/0000-0001-5579-7924
http://rave.ohiolink.edu/etdc/view?acc_num=osu1607035449218475

Abstract Details

2020, Master of Science, Ohio State University, Horticulture and Crop Science.
A goal of The Ohio State University Hordeum vulgare L. breeding program is to develop winter-hardy, two-row varieties suitable for malting. Winter-hardiness and malting quality are complex traits, having multiple independent contributing components. It is critical that the program identify genotypes possessing the best agronomic and malting quality traits to guarantee Ohio barley farmers are profitable. To make better informed decisions regarding variety selection for cultivation under Ohio conditions and guide parental choice for crossing, I conducted a genome wide association study (GWAS). GWAS analyzes phenotypic data and genotypic data to test for associations between the markers and desirable traits. The GWAS was conducted using 350 barley genotypes that encompassed key germplasm contributing to the breeding program. The germplasm set was genotyped and phenotyped for agronomic and malting quality traits. Genotyping was performed using multiplexed sequencing using markers based off the barley 9k chip in collaboration with the USDA Western Regional Small Grains Genotyping Lab. Phenotypic data was collected during the 2019-2020 growing season at Wooster, North Central, and Western research branches. I identified at least one QTL associated with 27 of the 31 traits measured and identified 78 QTLs in total. Identified QTLs were all novel to Ohio malting barley and some match genomic regions previously recorded in other environments. The significant markers can be used in marker assisted selection in future breeding efforts. The phenotypic correlations found will allow for more accurate characterization of new genetic material.
Stockinger Eric (Advisor)
Sneller Clay (Committee Member)
Barker David (Committee Member)
Genetics; Plant Sciences

Recommended Citations

Citations

  • Eggers, B. (2020). Identifying phenotypic traits critical for breeding winter malting barley adapted to Ohio and the genomic regions affecting those traits [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1607035449218475

    APA Style (7th edition)

  • Eggers, Ben. Identifying phenotypic traits critical for breeding winter malting barley adapted to Ohio and the genomic regions affecting those traits. 2020. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1607035449218475.

    MLA Style (8th edition)

  • Eggers, Ben. "Identifying phenotypic traits critical for breeding winter malting barley adapted to Ohio and the genomic regions affecting those traits." Master's thesis, Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1607035449218475

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.