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Phillip A Craigmile HTC Thesis.pdf (3.07 MB)

ETD Abstract Container

Abstract Header

The effects of ionizing radiation and p53 mutation on cancer cell migration and epithelial-mesenchymal transition (EMT)

Craigmile, Phillip A
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1461325343

Abstract Details

2016, Bachelor of Science (BS), Ohio University, Biological Sciences.
Metastasis is involved in 90% of cancer deaths. Ionizing radiation (IR), which is a common cancer treatment, has been shown to alter migration and invasive potential in multiple cancer cell lines in previous research. The tumor suppressor protein, p53, is mutated in about 50% cancers. This project investigated the role of p53 mutation in a cancer's migratory response and epithelial-mesenchymal transition (EMT) after treatment with a single dose of IR (4 Gy or 8 Gy) or with a fractionated treatment (4 treatments of 2 Gy). A single dose of 8 Gy increased migration in H1299 cells expressing p53-R280K and also slightly increased expression of EMT marker proteins. No other significant differences occurred after treatment with 4 Gy or 8 Gy. Fractionated treatment increased migration in H1299 p53-null cells, but did not alter migration or EMT in H1299 cells expressing p53-WT, p53-R175H, or p53-R280K.
Shiyong Wu, Ph.D. (Advisor)
61 p.
Biology; Radiation
ionizing radiation; migration; epithelial-mesenchymal transition; EMT; p53; p53 mutation; H1299

Recommended Citations

Citations

  • Craigmile, P. A. (2016). The effects of ionizing radiation and p53 mutation on cancer cell migration and epithelial-mesenchymal transition (EMT) [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1461325343

    APA Style (7th edition)

  • Craigmile, Phillip. The effects of ionizing radiation and p53 mutation on cancer cell migration and epithelial-mesenchymal transition (EMT). 2016. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1461325343.

    MLA Style (8th edition)

  • Craigmile, Phillip. "The effects of ionizing radiation and p53 mutation on cancer cell migration and epithelial-mesenchymal transition (EMT)." Undergraduate thesis, Ohio University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1461325343

    Chicago Manual of Style (17th edition)

ouhonors1461325343
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© 2016, all rights reserved.
This open access ETD is published by Ohio University Honors Tutorial College and OhioLINK.