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HTC_Thesis_2018_Caleb_Burke.pdf (14.28 MB)

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Identification and Analysis of Novel, Biofilm-Specific small RNAs (sRNAs) in Staphylococcus aureus

Burke, Caleb A, Burke
http://orcid.org/0000-0002-8628-7254
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1524837232775665

Abstract Details

2018, Bachelor of Science (BS), Ohio University, Biological Sciences.
In Staphylococcus aureus little is known about the regulation, function, or mechanism of action of most small RNAs (sRNAs). sRNA genes are typically absent from genome annotation files and, as a consequence, their existence is often overlooked, particularly in global transcriptomic studies. Recently we performed a study that catalogued and annotated sRNA genes on the genome of the S. aureus community-associated, methicillin resistant (MRSA) strain USA300 Houston. The newly created USA300 Genbank genome file is a valuable resource that facilitates improved detection and analysis of sRNAs in S. aureus. In this study, we performed an RNAseq of S. aureus USA300 Houston growing as a biofilm in order to (i) identify novel sRNAs that are uniquely expressed in a biofilm, (ii) to determine global transcription changes in known sRNAs during biofilm growth, and (iii) develop an assay to screen sRNAs for potential regulation of biofilm formation. This analysis led to the discovery of 19 previously unidentified sRNAs, biofilm specific RNA (bsr1-19), and 83 known sRNAs potentially involved in biofilm formation. Finally, we developed a crystal violet spectroscopy assay to quantify biofilm formation, and screened sRNAs for which we had a transposon mutants in our library.
Ronan Carroll, Dr. (Advisor)
66 p.
Biology; Genetics

Recommended Citations

Citations

  • Burke, Burke, C. A. (2018). Identification and Analysis of Novel, Biofilm-Specific small RNAs (sRNAs) in Staphylococcus aureus [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1524837232775665

    APA Style (7th edition)

  • Burke, Burke, Caleb. Identification and Analysis of Novel, Biofilm-Specific small RNAs (sRNAs) in Staphylococcus aureus. 2018. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1524837232775665.

    MLA Style (8th edition)

  • Burke, Burke, Caleb. "Identification and Analysis of Novel, Biofilm-Specific small RNAs (sRNAs) in Staphylococcus aureus." Undergraduate thesis, Ohio University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1524837232775665

    Chicago Manual of Style (17th edition)

ouhonors1524837232775665
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© 2018, all rights reserved.
This open access ETD is published by Ohio University Honors Tutorial College and OhioLINK.