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Senior Thesis_Puri, I_Final PDF.pdf (11.42 MB)

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IDENTIFYING FSP27 AS A NOVEL PLAYER IN MUSCLE PERFORMANCE

Puri, Ishika
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682694046524486

Abstract Details

2023, Bachelor of Science (BS), Ohio University, Biological Sciences.
Fat-specific protein 27 (FSP27) was originally identified as a lipid droplet-associated protein regulating fat metabolism in the adipose tissue. Whole-body Fsp27-/- mice have previously been shown to have higher oxidative metabolism. We identified that FSP27 is also expressed in skeletal muscle where it co-localizes with intra-muscular fat. We hypothesized that due to their high oxidative capacity, Fsp27-/- mice would have a high level of muscle endurance. To test our hypothesis, we performed metabolic phenotype and muscle endurance in cohorts of Wild-type, Fsp27+/- and Fsp27-/- male and female mice at 8-10 months of age. These mice displayed reduced muscle endurance accompanied by marked deficiencies in fat storage in their skeletal muscle. This unexpected discovery led us to think of two possibilities, a) the poor endurance was due to chronic higher oxidative metabolism which would gradually reduce fat content in the muscles or cause oxidative stress over time, or b) Fsp27 has a direct role in fat handling in the skeletal muscle. Therefore, we preformed muscle performance studies in mice at 5-6 months of age as well as 2-3 months of age, which are equivalent to approx. 20 yrs and 34 yrs in humans, respectively. We found that even at 2-months of age Fsp27-/- mice, displayed significantly reduced poor muscle performance similar5-6 months or 10 months old mice . Also, these mice had negligible fat in their skeletal muscles. Fat being a crucial energy source for enduring muscle, our results suggest that similar to its role in the adipose tissue, FSP27 has a direct role in fat storage in the skeletal muscle which plays an important role in muscle endurance. Overall, our studies highlight a previously unidentified role of Fsp27 in muscle performance.
Dr. Vishva M. Sharma (Advisor)
62 p.
Biology
CIDE-C, FSP27

Recommended Citations

Citations

  • Puri, I. (2023). IDENTIFYING FSP27 AS A NOVEL PLAYER IN MUSCLE PERFORMANCE [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682694046524486

    APA Style (7th edition)

  • Puri, Ishika. IDENTIFYING FSP27 AS A NOVEL PLAYER IN MUSCLE PERFORMANCE . 2023. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682694046524486.

    MLA Style (8th edition)

  • Puri, Ishika. "IDENTIFYING FSP27 AS A NOVEL PLAYER IN MUSCLE PERFORMANCE ." Undergraduate thesis, Ohio University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1682694046524486

    Chicago Manual of Style (17th edition)

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